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Jing Ying Yap, Ching Lik Hii, Sze Pheng Ong, Kuan Hon Lim, Faridah Abas, Kar Yong Pin. Quantification of Carpaine and antioxidant properties of extracts from Carica Papaya plant leaves and stalks[J]. Journal of Bioresources and Bioproducts. doi: 10.1016/j.jobab.2021.03.002
Citation: Jing Ying Yap, Ching Lik Hii, Sze Pheng Ong, Kuan Hon Lim, Faridah Abas, Kar Yong Pin. Quantification of Carpaine and antioxidant properties of extracts from Carica Papaya plant leaves and stalks[J]. Journal of Bioresources and Bioproducts. doi: 10.1016/j.jobab.2021.03.002

Quantification of Carpaine and antioxidant properties of extracts from Carica Papaya plant leaves and stalks

doi: 10.1016/j.jobab.2021.03.002
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  • Corresponding author: Corresponding author.Future Food Malaysia, Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia
    Email address: Ching-Lik.Hii@Nottingham.edu.my (Ching Lik Hii).
  • Received Date: 2020-09-22
  • Accepted Date: 2020-12-28
  • Rev Recd Date: 2020-12-20
  • Available Online: 2021-03-19
  • Significance increase in dengue cases have been recordedworldwide every year and South East Asian countries have been badly affected. Currently, antiviral drug to treat dengue is still not available but papaya leaves extract (PLE) have been successfully used in treating dengue patients. Carpaine in PLE is the major active compounds that contributes to the anti-thrombocytopenic activity (raising platelet count in patient's blood). The PLE also contains polyphenols that contribute to antioxidant properties. Studies were carried out to extract and quantify carpaine from young leaves, old leaves and stalks of papaya plant. Carpaine crystalline powders were successfully purified and confirmed by 1H and 13C NMR (Nuclear Magnetic Resonance). Young papaya leaves recorded significantly higher amount of carpaine (P < 0.05) as compared with old leaves and stalks. Blending treatment showed significant effect (P < 0.05) on the amount of carpaine extracted from the young and old leaves and blended young leaves showed significantly much higher amount of carpaine as compared with the unblended samples (P < 0.05). In terms of total polyphenols content (TPC), young leaves (both unblended and blended samples) contained significantly higher amount of TPC (P < 0.05) followed by the old leaves and stalks. Results also showed relationship between the TPC and the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay scavenging activities namely IC50 (R2 = 0.9743) and acid equivalent antioxidant capacity (AEAC) (R2 = 0.9581). Both young and old leaves also showed significantly higher (P < 0.05) DPPH scavenging activities as compared with the stalks in both unblended and blended samples. Young papaya leaves were recommended as source of material to extract carpaine for future development of drug in dengue treatment.

     

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  • [1]
    Abuajah, C.I., Ogbonna, A.C., Osuji, C.M., 2015. Functional components and medicinal properties of food: a review. J. Food Sci. Technol. 52, 2522-2529. doi: 10.1007/s13197-014-1396-5
    [2]
    Ahmad, N., Fazal, H., Ayaz, M., Abbasi, B.H., Mohammad, I., Fazal, L., 2011. Dengue fever treatment with Carica papaya leaves extracts. Asian Pac. J. Trop. Biomed. 1, 330-333. http://europepmc.org/articles/PMC3614241
    [3]
    Ahmad, R., Suzilah, I., Wan Najdah, W.M.A., Topek, O., Mustafakamal, I., Lee, H.L., 2018. Factors determining dengue outbreak in Malaysia. PLoS One13, e0193326. DOI: 10.1371/journal.pone.0193326.
    [4]
    Anwar, K., Rahmanto, B., Triyasmono, L., Rizki, M.I., Halwany, W., Lestari, F., 2017. The influence of leaf age on total phenolic, flavonoids, and free radical scavenging capacity of Aquilaria beccariana. Res. J. Pharm. Biol. Chem. Sci. 8, 129-133. http://www.researchgate.net/publication/313435961_The_Influence_of_Leaf_Age_on_Total_Phenolic_Flavonoids_and_Free_Radical_Scavenging_Capacity_of_Aquilaria_beccariana
    [5]
    Becker, L., Zaiter, A., Petit, J., Zimmer, D., Karam, M.C., Baudelaire, E., Scher, J., Dicko, A., 2016. Improvement of antioxidant activity and polyphenol content of Hypericum perforatum and Achillea millefolium powders using successive grinding and sieving. Ind. Crop. Prod. 87, 116-123. doi: 10.1016/j.indcrop.2016.04.036
    [6]
    Bujang, M.A., Mudin, R.N., Haniff, J., Ikhwan, T.M., Pin, G.P., 2017. Trend of dengue infection in Malaysia and the forecast up until year 2040. Int. Med. J. 24, 438-441. http://www.researchgate.net/publication/321681579_Trend_of_dengue_infection_in_Malaysia_and_the_forecast_up_until_year_2040
    [7]
    Della Valle, A., Dimmito, M.P., Zengin, G., Pieretti, S., Mollica, A., Locatelli, M., Cichelli, A., Novellino, E., Ak, G., Yerlikaya, S., Baloglu, M.C., Celik Altunoglu, Y., Stefanucci, A., 2020. Exploring the nutraceutical potential of dried pepper Capsicum annuum L. on market from altino in abruzzo region. Antioxidants9, 400. doi: 10.3390/antiox9050400
    [8]
    Dharmarathna, S.L.C.A., Wickramasinghe, S., Waduge, R.N., Rajapakse, R.P.V.J., Kularatne, S.A.M., 2013. Does Carica papaya leaf-extract increase the platelet count? An experimental study in a murine model. Asian Pac. J. Trop. Biomed. 3, 720-724. doi: 10.1016/S2221-1691(13)60145-8
    [9]
    Itidel, C., Chokri, M., Mohamed, B., Yosr, Z., 2013. Antioxidant activity, total phenolic and flavonoid content variation among Tunisian natural populations of Rhus tripartita (Ucria) Grande and Rhus pentaphylla Desf. Ind. Crop. Prod. 51, 171-177. doi: 10.1016/j.indcrop.2013.09.002
    [10]
    Kaysar, M.I., Hoq, M.S., Islam, M.W., Islam, M.S., Islam, M.T., 2019. Profitability analysis of Papaya cultivation in some selected areas of Bangladesh. Bangladesh J. Agric. Res. 44, 115-126. doi: 10.3329/bjar.v44i1.40936
    [11]
    Ketsuwan, N., Leelarungrayub, J., Kothan, S., Singhatong, S., 2017. Antioxidant compounds and activities of the stem, flower, and leaf extracts of the anti-smoking Thai medicinal plant: Vernonia cinerea Less. Drug Des. Dev. Ther. 11, 383-391. doi: 10.2147/DDDT.S126882
    [12]
    Lim, Y.Y., Lim, T.T., Tee, J.J., 2007. Antioxidant properties of several tropical fruits: a comparative study. Food Chem. 103, 1003-1008. doi: 10.1016/j.foodchem.2006.08.038
    [13]
    Mudin, R.N., 2015. Dengue incidence and the prevention and control program in Malaysia. IIUM Med. J. Malays. 14: 5-9. http://search.ebscohost.com/login.aspx?direct=true&db=aph&AN=103242147&site=ehost-live
    [14]
    Sagar, V.R., Suresh Kumar, P., 2010. Recent advances in drying and dehydration of fruits and vegetables: a review. J. Food Sci. Technol. 47, 15-26. doi: 10.1007/s13197-010-0010-8
    [15]
    Santana, L.F., Inada, A.C., Espirito Santo, B.L.S.D., Filiú, W.F.O., Pott, A., Alves, F.M., de Cássia A Guimarães, R., de Cássia Freitas, K., Hiane, P.A., 2019. Nutraceutical potential of Carica papaya in metabolic syndrome. Nutrients 11, 1608. doi: 10.3390/nu11071608
    [16]
    Santhanam Menon, A., Hii, C.L., Law, C.L., Shariff, S., Djaeni, M., 2017. Effects of drying on the production of polyphenol-rich cocoa beans. Dry. Technol. 35, 1799-1806. doi: 10.1080/07373937.2016.1276072
    [17]
    Sato, T., Aoyagi, S., Kibayashi, C., 2003. Enantioselective total synthesis of (+)-azimine and (+)-carpaine. Org. Lett. 5, 3839-3842. doi: 10.1021/ol030088w
    [18]
    Shih, M.C., Chang, C.M., Kang, S.M., Tsai, M.L., 2011. Effect of different parts (leaf, stem and stalk) and seasons (summer and winter) on the chemical compositions and antioxidant activity of Moringa oleifera. Int. J. Mol. Sci. 12, 6077-6088. doi: 10.3390/ijms12096077
    [19]
    Shubham, S., Mishra, R., Gautam, N., Nepal, M., Kashyap, N., Dutta, K., 2019. Phytochemical analysis of papaya leaf extract: screening test. EC Dental Sci. 18, 485-490. http://www.researchgate.net/publication/331641625_Phytochemical_Analysis_of_Papaya_Leaf_Extract_Screening_Test/download
    [20]
    Singh, Y., Bhatnagar, P., 2019. A review on exploitation of nutraceuticals from potential fruit crops. Int. J. Pure App. Biosci. 7, 213-223. doi: 10.18782/2320-7051.7422
    [21]
    Stefanucci, A., Zengin, G., Llorent-Martinez, E.J., Dimmito, M.P., Della Valle, A., Pieretti, S., Ak, G., Sinan, K.I., Mollica, A, 2020. Chemical characterization, antioxidant properties and enzyme inhibition of Rutabaga root's pulp and peel (Brassica napus L. ). Arab. J. Chem. 13, 7078-7086. doi: 10.1016/j.arabjc.2020.07.013
    [22]
    Subenthiran, S., Choon, T.C., Cheong, K.C., Thayan, R., Teck, M.B., Muniandy, P.K., Afzan, A., Abdullah, N.R., Ismail, Z., 2013. Carica papaya leaves juice significantly accelerates the rate of increase in platelet count among patients with dengue fever and dengue haemorrhagic fever. Evid. -Based Complementary Altern. Med. 2013, 1-7.
    [23]
    Teixeira, R., Pereira, W., Oliveira, A., da Silva, A., de Oliveira, A., da Silva, M., da Silva, C., de Paula, S., 2014. Natural products as source of potential dengue antivirals. Molecules19, 8151-8176. doi: 10.3390/molecules19068151
    [24]
    Thi, N.D., Hwang, E.S., 2014. Bioactive compound contents and antioxidant activity in Aronia (Aronia melanocarpa) leaves collected at different growth stages. Prev. Nutr. Food Sci. 19, 204-212. doi: 10.3746/pnf.2014.19.3.204
    [25]
    World Health Organization (WHO), 2020. Dengue and Severe Dengue. Available at: https://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue (accessed 18 March 2020).
    [26]
    Yap, J.Y., Hii, C.L., Ong, S.P., Lim, K.H., Abas, F., Pin, K.Y., 2020. Effects of drying on total polyphenols content and antioxidant properties of Carica papaya leaves. J. Sci. Food Agric. 100, 2932-2937. doi: 10.1002/jsfa.10320
    [27]
    Zehiroglu, C., Ozturk Sarikaya, S.B., 2019. The importance of antioxidants and place in today's scientific and technological studies. J. Food Sci. Technol. 56, 4757-4774. doi: 10.1007/s13197-019-03952-x
    [28]
    Zunjar, V., Dash, R.P., Jivrajani, M., Trivedi, B., Nivsarkar, M., 2016. Antithrombocytopenic activity of carpaine and alkaloidal extract of Carica papaya Linn. leaves in busulfan induced thrombocytopenic Wistar rats. J. Ethnopharmacol. 181, 20-25. doi: 10.1016/j.jep.2016.01.035
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