Volume 6 Issue 4
Oct.  2021
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D. Menaga, Pattanathu K.S.M. Rahman, S. Rajakumar, P.M. Ayyasamy. Antioxidant and Cytotoxic Activities of A Novel Isomeric Molecule (PF5) Obtained from Methanolic Extract of Pleurotus Florida Mushroom[J]. Journal of Bioresources and Bioproducts, 2021, 6(4): 338-349. doi: 10.1016/j.jobab.2021.04.008
Citation: D. Menaga, Pattanathu K.S.M. Rahman, S. Rajakumar, P.M. Ayyasamy. Antioxidant and Cytotoxic Activities of A Novel Isomeric Molecule (PF5) Obtained from Methanolic Extract of Pleurotus Florida Mushroom[J]. Journal of Bioresources and Bioproducts, 2021, 6(4): 338-349. doi: 10.1016/j.jobab.2021.04.008

Antioxidant and Cytotoxic Activities of A Novel Isomeric Molecule (PF5) Obtained from Methanolic Extract of Pleurotus Florida Mushroom

doi: 10.1016/j.jobab.2021.04.008
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  • Corresponding author: E-mail address: pmayyasamy@gmail.com (P.M. Ayyasamy)
  • Received Date: 2020-08-05
  • Accepted Date: 2020-11-15
  • Rev Recd Date: 2020-11-08
  • Publish Date: 2021-11-01
  • The Pleurotus florida is recognized as a medicinal and edible mushroom and the present study intends to reveal the active isomeric molecules from this mushroom. The P. florida was cultivated using different nutrient supplements: groundnut husk, maize powder, horse gram powder and coconut oil-cake powder. Horse gram supplement showed the higher mushroom yield and henceit was used for the cultivation of P. florida. Methanolic extract of P. florida was found to be efficient in antioxidant activity among ethanol, aqueous, ethyl acetate and hexane extracts. The bioactive fraction 3-methoxy-4-hydroxy cinnamic acid (PF5) was isolated and purified from the methanolic extract of P. florida by column chromatography, thin layer chromatography (TLC) and gas chromatography-mass spectrum (GC-MS) and further it was characterized by Nuclear magnetic resonance (NMR). The PF5 was tested for its DPPH and reducing power assays, and the IC50 values were found to be 21.7 µg/mL and 105 µg/mL, respectively. We found that the cytotoxic effect of 3-methoxy-4-hydroxy cinnamic acid was tested against the lung cancer cell line using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT), sulphorodamine B (SRB) and trypan blue assays which exhibited a higher cytotoxic effect (CTC50, 645 µg/mL). These results suggested that 3-methoxy-4-hydroxy cinnamic acid from P. florida could be explored as a novel and potent natural antioxidant and cancer preventive agent, alternative to existing synthetic molecules.


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  • Bergendiova, K., Tibenska, E., Majtan, J., 2011. Pleuran (β-glucan from Pleurotus ostreatus) supplementation, cellular immune response and respiratory tract infections in Athletes. Eur. J. Appl. Physiol. 111, 2033-2040 doi: 10.1007/s00421-011-1837-z
    Berker, K., Güçlü, K., Tor, İ., Demirata, B., Apak, R., 2010. Total antioxidant capacity assay using optimized ferricyanide/Prussian blue method. Food Anal. Methods 3, 154-168
    Bhat, R., Sharanabasava, V.G., Deshpande, R., Shetti, U., Sanjeev, G., Venkataraman, A., 2013. Photo-bio-synthesis of irregular shaped functionalized gold nanoparticles using edible mushroom Pleurotus Florida and its anticancer evaluation. J. Photochem. Photobiol. B: Biol. 125, 63-69 doi: 10.4103/2347-5633.157885
    Bhunia, S.K., Dey, B., Maity, K.K., Patra, S., Mandal, S., Maiti, S., Maiti, T.K., Sikdar, S.R., Islam, S.S., 2012. Heteroglycan from an alkaline extract of a somatic hybrid mushroom (PfloVv1aFB) of Pleurotus Florida and Volvariella volvacea: structural characterization and study of immunoenhancing properties. Carbohydr. Res. 354, 110-115
    Chen, Y.S., Huang, J.Q., Hu, J., Yan, R.A., Ma, X., 2019. Comparative study on the phytochemical profiles and cellular antioxidant activity of phenolics extracted from barley malts processed under different roasting temperatures. Food Funct 10, 2176-2185
    Choi, J.H., Yoon, S.K., Lee, K.H., Seo, M.S., Kim, D.H., Hong, S.B., Kim, J.Y., Paik, H.D., Kim, C.H., 2006. Antitumor activity of cell suspension culture of green tea seed (Camellia sinensis L. ). Biotechnol. Bioprocess Eng. 11, 396 doi: 10.1109/ECWT.2006.280521
    Denizot, F., Lang, R.T., 1986. Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 89, 271-277
    Dey, B., Bhunia, S.K., Maity, K.K., Patra, S., Mandal, S., Behera, B., Maiti, T.K., Sikdar, S.R., Islam, S.S., 2013. Structure and immunology of a heteroglycan from somatic hybrid mushroom of Pleurotus Florida and Calocybe indica var. APK2. Int. J. Biol. Macromol. 52, 312-318
    Doğan, H.H., Akbaş, G., 2013. Biological activity and fatty acid composition of Caesar's mushroom. Pharm. Biol. 51, 863-871 doi: 10.3109/13880209.2013.768272
    Fazary, A.E., Ju, Y.H., 2007. Feruloyl esterases as biotechnological tools: current and future perspectives. Acta Biochim Biophys Sin (Shanghai) 39, 811-828 doi: 10.1111/j.1745-7270.2007.00348.x
    Finimundy, T.C., Gambato, G., Fontana, R., Camassola, M., Salvador, M., Moura, S., Hess, J., Henriques, J.A.P., Dillon, A.J.P., Roesch-Ely, M., 2013. Aqueous extracts of Lentinula edodes and Pleurotus sajor-caju exhibit high antioxidant capability and promising in vitro antitumor activity. Nutr. Res. 33, 76-84
    Ganeshpurkar, A., Bhadoriya, S.S., Pardhi, P., Jain, A.P., Rai, G., 2011. In vitro prevention of cataract by oyster mushroom Pleurotus florida extract on isolated goat eye lens. Indian J. Pharmacol. 43, 667-670
    Janardhanan, K.K., Jose, N., Janardhanan, K.K., 2000. Antioxidant and antitumor activity of Pleurotus florida. Curr. Sci. 79, 941-943
    Jose, N., Ajith, T.A., Janardhanan, K.K., 2004. Methanol extract of the oyster mushroom, Pleurotus Florida, inhibits inflammation and platelet aggregation. Phytother. Res. 18, 43-46
    Khan, M.A., 2011. Pleurotus sajor-caju and Pleurotus florida mushrooms improve some extent of the antioxidant systems in the liver of hypercholesterolemic rats. Open Nutraceuticals J 4, 20-24 doi: 10.2174/1876396001104010020
    Khodabande, Z., Jafarian, V., Sariri, R., 2017. Antioxidant activity of Chelidonium majus extract at phenological stages. Appl. Biol. Chem. 60, 497-503 doi: 10.1007/s13765-017-0304-x
    Kong, B.H., Tan, N.H., Fung, S.Y., Pailoor, J., Tan, C.S., Ng, S.T., 2016. Nutritional composition, antioxidant properties, and toxicology evaluation of the sclerotium of Tiger Milk Mushroom Lignosus tigris cultivar E. Nutr. Res. 36, 174-183
    Kong, W.S., 2004. Descriptions of commercially important Pleurotus species. Mushroom Growers' Handbook 1. Oyster Mushroom Cultivation, pp. 54–61 Mushworld 2004.
    Liu, J., Jia, L., Kan, J., Jin, C.H., 2013. In vitro and in vivo antioxidant activity of ethanolic extract of white button mushroom (Agaricus bisporus). Food Chem. Toxicol. 51, 310-316 doi: 10.3724/SP.J.1123.2012.12005
    Mabinya, L.V., Mafunga, T., Brand, J.M., 2006. Determination of ferulic acid and related compounds by thin layer chromatography. African J. Biotechnol. 5, 1271-1273
    Maity, G.N., Maity, P., Choudhuri, I., Bhattacharyya, N., Acharya, K., Dalai, S., Mondal, S., 2019. Structural studies of a water insoluble β-glucan from Pleurotus djamor and its cytotoxic effect against PA1, ovarian carcinoma cells. Carbohydr. Polym. 222, 114990
    Maity, K., Kar (Mandal), E., Maity, S., Gantait, S.K., Das, D., Maiti, S., Maiti, T.K., Sikdar, S.R., Islam, S.S., 2011. Structural characterization and study of immunoenhancing and antioxidant property of a novel polysaccharide isolated from the aqueous extract of a somatic hybrid mushroom of Pleurotus florida and Calocybe indica variety APK2. Int. J. Biol. Macromol. 48, 304-310
    Maity, S., Bhunia, S.K., Sen, I.K., Mandal, E.K., Maity, K., Behera, B., Maiti, T.K., Mallick, P., Sikdar, S.R., Islam, S.S., 2013. A heteropolysaccharide from an edible hybrid mushroom pfle 1p: structural and immunostimulating studies. Carbohydr. Res. 374, 89-95
    Menaga, D., Mahalingam, P.U., Rajakumar, S., Ayyasamy, P.M., 2012. Evaluation of phytochemical characteristics and antimicrobial activity of Pleurotus florida mushroom. Asian J. Pharm. Clin. Res. 5, 102-106
    Menaga, D., Rajakumar, S., Ayyasamy, P.M., 2013. Free radical scavenging activity of methanolic extract of Pleurotus florida mushroom. Int. J. Pharmacy Pharm. Sci 5, 601-606
    Molyneux, P., 2004. The use of the stable free radical diphenyl picrylhydrazyl (DPPH) for estimating antioxidant activity. J. Sci. Educ. Technol. 26, 211-219
    Nasehi, M., Torbatinejad, N.M., Zerehdaran, S., Safaie, A.R., 2017. Effect of solid-state fermentation by oyster mushroom (Pleurotus florida) on nutritive value of some agro by-products. J. Appl. Animal Res. 45, 221-226 doi: 10.1080/09712119.2016.1150850
    Owaid, M.N., 2019. Green synthesis of silver nanoparticles by Pleurotus (oyster mushroom) and their bioactivity: Review. Environ. Nanotechnol. Monit. Manag. 12, 100256
    Prabhakar, P.K., Doble, M., 2011. Interaction of cinnamic acid derivatives with commercial hypoglycemic drugs on 2-deoxyglucose uptake in 3T3-L1 adipocytes. J. Agric. Food Chem. 59, 9835-9844 doi: 10.1021/jf2015717
    Prashar, Y., Patel, N.J., 2020. An in vitro approach to evaluate the anti-adipogenic effect of Myrica nagi Thunb. Fruit extract on 3T3-L1 adipocyte cell line. Obes. Med. 18, 100228
    Rana, T., Bera, A.K., Das, S., Bhattacharya, D., Pan, D., Bandyopadhyay, S., Mondal, D.K., Samanta, S., Bandyopadhyay, S., Das, S.K., 2012. Pleurotus florida lectin normalizes duration dependent hepatic oxidative stress responses caused by arsenic in rat. Exp. Toxicol. Pathol. 64, 665-671
    Selvi, S., Umadevi, P., Murugan, S., GiftsonSenapathy, J., 2011. Anticancer potential evoked by Pleurotus florida and Calocybe indica using T24 urinary bladder cancer cell line. African J. Biotechnol. 10, 7279-7285
    Siddhuraju, P., Mohan, P.S., Becker, K., 2002. Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L. ): a preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp. Food Chem 79, 61-67
    Singh, M., Vijay, B., Kamal, S., Wakchaure, G.C., 2011. Mushrooms: cultivation, marketing and consumption, directorate of mushroom research. Indian Council of Agricultural Research (ICAR), Solan, India.
    Skehan, P., Storeng, R., Scudiero, D., Monks, A., McMahon, J., Vistica, D., Warren, J.T., Bokesch, H., Kenney, S., Boyd, M.R., 1990. New colorimetric cytotoxicity assay for anticancer-drug screening. JNCI J. Natl. Cancer Inst. 82, 1107-1112 doi: 10.1093/jnci/82.13.1107
    Song, F.Q., Liu, Y., Kong, X.S., Chang, W., Song, G., 2013. Progress on understanding the anticancer mechanisms of medicinal mushroom: Inonotus obliquus. Asian Pac. J. Cancer Prev. 14, 1571-1578
    Sreerama, Y.N., Neelam, D.A., Sashikala, V.B., Pratape, V.M., 2010. Distribution of nutrients and antinutrients in milled fractions of chickpea and horse gram: seed coat phenolics and their distinct modes of enzyme inhibition. J. Agric. Food Chem. 58, 4322-4330 doi: 10.1021/jf903101k
    Sreerama, Y.N., Sashikala, V.B., Pratape, V.M., 2010. Variability in the distribution of phenolic compounds in milled fractions of chickpea and horse gram: evaluation of their antioxidant properties. J. Agric. Food Chem. 58, 8322-8330 doi: 10.1021/jf101335r
    Sreerama, Y.N., Sashikala, V.B., Pratape, V.M., 2012. Phenolic compounds in cowpea and horse gram flours in comparison to chickpea flour: evaluation of their antioxidant and enzyme inhibitory properties associated with hyperglycemia and hypertension. Food Chem 133, 156-162
    Stahl, E., 1969. Thin Layer Chromatography, 2nd ed. Springer-Verlag, New York.
    Sumy, A.K., Jahan, N., Sultana, N., 1970. Study on the hepatoprotective effect of oyster mushroom (Pleurotus florida) against paracetamol induced liver damage in wistar albino rats. J. Bangladesh Soc. Physiol. 5, 46-52 doi: 10.3329/jbsp.v5i2.6776
    Unnikrishnan, M.C., Kuttan, R., 1988. Cytotoxicity of extracts of spices to cultured cells. Nutr. Cancer 11, 251-257 doi: 10.1080/01635588809513995
    van den Worm, E., Beukelman, C.J., van den Berg, A.J.J., Kroes, B.H., Labadie, R.P., van Dijk, H., 2001. Effects of methoxylation of apocynin and analogs on the inhibition of reactive oxygen species production by stimulated human neutrophils. Eur. J. Pharmacol. 433, 225-230
    Venturella, G., Gargano, M.L., 2017. Medicinal properties of mediterranean oyster mushrooms: species of genus pleurotus (higher basidiomycetes). Med. Plants Fungi: Recent Adv. Res. Dev. 4, 49-58 doi: 10.1007/978-981-10-5978-0_2
    Vera-Ramirez, L., Sanchez-Rovira, P., Ramirez-Tortosa, M.C., Ramirez-Tortosa, C.L., Granados-Principal, S., Lorente, J.A., Quiles, J.L., 2011. Free radicals in breast carcinogenesis, breast cancer progression and cancer stem cells. Biological bases to develop oxidative-based therapies. Crit. Rev. Oncol. 80, 347-368
    Xie, C.Y., Gu, Z.X., You, X.J., Liu, G.X., Tan, Y.X., Zhang, H., 2010. Screening of edible mushrooms for release of ferulic acid from wheat bran by fermentation. Enzym. Microb. Technol. 46, 125-128
    Zhao, Q.S., Xie, B.X., Yan, J., Zhao, F.C., Xiao, J., Yao, L.Y., Zhao, B., Huang, Y.X., 2012. In vitro antioxidant and antitumor activities of polysaccharides extracted from Asparagus officinalis. Carbohydr. Polym. 87, 392-396
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