Ameliorated enzymatic saccharification of corn stover with a novel modified alkali pretreatment

Guang Yu; Huanfei Xu; Chao Liu; Paul DeRoussel; Chunyan Zhang; Yuedong Zhang; Bin Li; Haisong Wang; Xindong Mu

doi:10.21967/jbb.v1i1.36

Ameliorated enzymatic saccharification of corn stover with a novel modified alkali pretreatment

doi: 10.21967/jbb.v1i1.36

a. CAS Key Laboratory of Bio-based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China;
b. College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;
c. Procter and Gamble Manufacturing, Procter and Gamble Co., Mason, Ohio, 45040, USA;
d. Materials, Process and Delivery, Procter and Gamble Singapore Innovation Centre, Procter and Gamble Co., Singapore, 138648, Singapore

基金项目:

The authors are grateful for the great support of research funding from Procter and Gamble Co. This work was also partially supported by the National Natural Science Foundation of China (Grant No.31370582, Grant No. 21306216, and Grant No. 31470609), Shandong Provincial Natural Science Foundation for Distinguished Young Scholar (China) (Grant No. JQ201305), as well as the National High Technology Research and Development Program ("863" program) of China (Grant No. 2012AA022301).

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出版历程

Ameliorated enzymatic saccharification of corn stover with a novel modified alkali pretreatment

a. CAS Key Laboratory of Bio-based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China;
b. College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;
c. Procter and Gamble Manufacturing, Procter and Gamble Co., Mason, Ohio, 45040, USA;
d. Materials, Process and Delivery, Procter and Gamble Singapore Innovation Centre, Procter and Gamble Co., Singapore, 138648, Singapore

Funds:

摘要

摘要: Enzymatic saccharification/hydrolysis is one of the key steps for the bioconversion of lignocelluloses into sustainable biofuels. In this work, corn stover was pretreated with a novel modified alkali process (NaOH + anthraquinone (AQ) + sodium lignosulfonate (SLS)), and then enzymatically hydrolyzed with an enzyme cocktail (cellulase (Celluclast 1.5L), β-glucosidase (Novozyme 188) and xylanase (from thermomyceslanuginosus)) in the pH range of 4.0-6.5. It was found that the suitable pH for the enzymatic saccharification process to achieve a high glucan yield was between 4.2 and 5.7, while the appropriate pH to obtain a high xylan yield was in the range of 4.0-4.7. The best pH for the enzymatic saccharification process was found to be 4.4 in terms of the final total sugar yield, as xylanase worked most efficiently in the pH range of 4.0-4.7, under the conditions in the study. The addition of xylanase in the enzymatic saccharification process could hydrolyze xylan in the substrates and reduce the nonspecific binding of cellulase, thus improving the total sugar yields.
- Enzymatic saccharification /
- Hydrolysis pH /
- Xylanase /
- Corn stover /
- Biorefinery /
- Fermentable sugar /
- cellulase
Abstract: Enzymatic saccharification/hydrolysis is one of the key steps for the bioconversion of lignocelluloses into sustainable biofuels. In this work, corn stover was pretreated with a novel modified alkali process (NaOH + anthraquinone (AQ) + sodium lignosulfonate (SLS)), and then enzymatically hydrolyzed with an enzyme cocktail (cellulase (Celluclast 1.5L), β-glucosidase (Novozyme 188) and xylanase (from thermomyceslanuginosus)) in the pH range of 4.0-6.5. It was found that the suitable pH for the enzymatic saccharification process to achieve a high glucan yield was between 4.2 and 5.7, while the appropriate pH to obtain a high xylan yield was in the range of 4.0-4.7. The best pH for the enzymatic saccharification process was found to be 4.4 in terms of the final total sugar yield, as xylanase worked most efficiently in the pH range of 4.0-4.7, under the conditions in the study. The addition of xylanase in the enzymatic saccharification process could hydrolyze xylan in the substrates and reduce the nonspecific binding of cellulase, thus improving the total sugar yields.
- Enzymatic saccharification /
- Hydrolysis pH /
- Xylanase /
- Corn stover /
- Biorefinery /
- Fermentable sugar /
- cellulase