Optimization of the extraction process for flavonoids from basil (<i>Ocimum basilicum</i>) using response surface methodology

Lei Wu; Ju-Wu Hu; Wei Xiong; Xiong-Hui Li; Guan-Hua Wang; Lin Dai; Young-Soo Bae; Chuan-Ling Si; Wei-Cheng Hu

doi:10.21967/jbb.v1i4.61

Optimization of the extraction process for flavonoids from basil (Ocimum basilicum) using response surface methodology

doi: 10.21967/jbb.v1i4.61

a. Institute of Applied Chemistry, Jiangxi Academy of Sciences, Nanchang 330096, China;
b. Tianjin Key Laboratory of Pulp and Paper, Tianjin University of Science and Technology, Tianjin 300457, China;
c. Jiangsu Province Biomass Energy and Materials Laboratory, Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, China;
d. Jiangsu Provincial Key Laboratory of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, P. R. China;
e. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China;
f. Department of Forest Biomaterials Engineering, College of Forest & Environment Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea;
g. Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental protection/Jiangsu Key Laboratory for Eco-agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian 223300, China

基金项目:

This project was financially supported by National Natural Science Foundation of China (31260400), the foundations of Jiangsu Province Biomass Energy and Materials Laboratory in Institute of Chemical Industry of Forest Products, CAF (JSBEM201601), State Key Laboratory of Pulp & Paper Engineering (201611, 201503), and Jiangsu Provincial Key Laboratory of Pulp and Paper Science and Technology, Nanjing Forestry University (201531), P.R. China, Natural Science Foundation of Tianjin City (16JCQNJC05900) for financial support.

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出版历程

Optimization of the extraction process for flavonoids from basil (Ocimum basilicum) using response surface methodology

Lei Wu^a,b,
Ju-Wu Hu^a,
Wei Xiong^a,
Xiong-Hui Li^a,
Guan-Hua Wang^b,e,
Lin Dai^b,
Young-Soo Bae^f,
Chuan-Ling Si^{b,c,d,e
, ,},
Wei-Cheng Hu^{g
, ,}

a. Institute of Applied Chemistry, Jiangxi Academy of Sciences, Nanchang 330096, China;
b. Tianjin Key Laboratory of Pulp and Paper, Tianjin University of Science and Technology, Tianjin 300457, China;
c. Jiangsu Province Biomass Energy and Materials Laboratory, Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, China;
d. Jiangsu Provincial Key Laboratory of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, Jiangsu Province, P. R. China;
e. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China;
f. Department of Forest Biomaterials Engineering, College of Forest & Environment Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea;
g. Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental protection/Jiangsu Key Laboratory for Eco-agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian 223300, China

Funds:

摘要

摘要: Basil (Ocimum basilicum), an edible and medicinal plant with high nutritional value and therapeutic efficacy, was used as a potential source of total flavonoids in this study. Heat reflux extractions were performed using aqueous ethanol. The optimized extraction conditions of total flavonoids from O. basilicum were determined by Box-Behnken design with response surface methodology. Response surface plots showed that the optional four independent variables significantly influenced the extraction yield of total flavonoids. The extraction parameters for the highest total flavonoids yield were optimized as:extraction temperature of 79.74℃, ethanol concentration of 77.63%, ratio of liquid to material of 29.72:1 (mL/g), and extraction time of 2.06 h. The average yield of total flavonoids under above optimum parameters was 42.61 mg of rutin equivalents per g of extract dry matter, which was in good agreement with the predicted value of 40.23 mg/g. These optimized conditions could be useful for the extraction of flavonoids from O. basilicum.
- Ocimum basilicum /
- total flavonoids /
- extraction /
- optimization /
- response surface methodology
Abstract: Basil (Ocimum basilicum), an edible and medicinal plant with high nutritional value and therapeutic efficacy, was used as a potential source of total flavonoids in this study. Heat reflux extractions were performed using aqueous ethanol. The optimized extraction conditions of total flavonoids from O. basilicum were determined by Box-Behnken design with response surface methodology. Response surface plots showed that the optional four independent variables significantly influenced the extraction yield of total flavonoids. The extraction parameters for the highest total flavonoids yield were optimized as:extraction temperature of 79.74℃, ethanol concentration of 77.63%, ratio of liquid to material of 29.72:1 (mL/g), and extraction time of 2.06 h. The average yield of total flavonoids under above optimum parameters was 42.61 mg of rutin equivalents per g of extract dry matter, which was in good agreement with the predicted value of 40.23 mg/g. These optimized conditions could be useful for the extraction of flavonoids from O. basilicum.
- Ocimum basilicum /
- total flavonoids /
- extraction /
- optimization /
- response surface methodology