Identification and characterization of slime-producing microorganisms in papermaking water loops using emulsion polymerase chain reaction techniques

Liuxin Shi; Gang Shi; Rongxia ZhuGe; Qiang Cheng; Fangfang Wu; Yonghao Ni

doi:10.21967/jbb.v3i3.176

Identification and characterization of slime-producing microorganisms in papermaking water loops using emulsion polymerase chain reaction techniques

doi: 10.21967/jbb.v3i3.176

a. Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China;
b. Limerick Pulp and Paper Centre, University of New Brunswick, Fredericton, New Brunswick, Canada E3B 5A3;
c. Key Laboratory of Forest Genetics & Biotechnology, MED, Nanjing Forestry University, Nanjing, 210037, China;
d. Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing, 210037, China

基金项目:

This work was supported by grants from the Major Research Project of the Natural Science Foundation of Jiangsu Higher Education Institutions (No. 14KJA53002), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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出版历程

Identification and characterization of slime-producing microorganisms in papermaking water loops using emulsion polymerase chain reaction techniques

a. Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China;
b. Limerick Pulp and Paper Centre, University of New Brunswick, Fredericton, New Brunswick, Canada E3B 5A3;
c. Key Laboratory of Forest Genetics & Biotechnology, MED, Nanjing Forestry University, Nanjing, 210037, China;
d. Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing, 210037, China

Funds:

摘要

摘要: Slime formation on paper machines is a critical issue that can substantially impact the quantity and quality of paper production. This problem is caused by the growth of an abundant and diverse amount of bacteria. Through the application of emulsion polymerase chain reaction (emPCR), the bacterial diversity was analyzed on paper machines and more operational taxonomic units (OTUs) were obtained. Eleven types of bacterial phyla were found that have been previously identified, including Proteobacteria (α-, β-, γ-, ε-, and φ-), Bacteroidetes, Firmicutes, Cyanobacteria, Verrucomicrobia, Actinobacteria, Spirochaetes, Chloroflexi, Deinococcus-Thermus, and Armatimonadetes. Furthermore, for the first time, there were representatives of the phyla Lentisphaerae found on paper machines. This study revealed the wide bacterial diversities of slime found on paper machines in China, which was also similar to other industrial processes.
- Slime /
- emPCR /
- OTUs /
- Diversity of bacteria /
- Bacterial phylum
Abstract: Slime formation on paper machines is a critical issue that can substantially impact the quantity and quality of paper production. This problem is caused by the growth of an abundant and diverse amount of bacteria. Through the application of emulsion polymerase chain reaction (emPCR), the bacterial diversity was analyzed on paper machines and more operational taxonomic units (OTUs) were obtained. Eleven types of bacterial phyla were found that have been previously identified, including Proteobacteria (α-, β-, γ-, ε-, and φ-), Bacteroidetes, Firmicutes, Cyanobacteria, Verrucomicrobia, Actinobacteria, Spirochaetes, Chloroflexi, Deinococcus-Thermus, and Armatimonadetes. Furthermore, for the first time, there were representatives of the phyla Lentisphaerae found on paper machines. This study revealed the wide bacterial diversities of slime found on paper machines in China, which was also similar to other industrial processes.
- Slime /
- emPCR /
- OTUs /
- Diversity of bacteria /
- Bacterial phylum