Optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels

Ude Michael U.; Oluka Ike; Eze Paul C.

doi:10.1016/j.jobab.2020.10.007

Volume 5 Issue 4

Nov. 2020

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Ude Michael U., Oluka Ike, Eze Paul C.. Optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels[J]. Journal of Bioresources and Bioproducts, 2020, 5(4): 283-290. doi: 10.1016/j.jobab.2020.10.007

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Ude Michael U., Oluka Ike, Eze Paul C.. Optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels[J]. Journal of Bioresources and Bioproducts, 2020, 5(4): 283-290. doi: 10.1016/j.jobab.2020.10.007

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Optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels

doi: 10.1016/j.jobab.2020.10.007

a.
Engineering Research Development and Production, Projects Development Institute(PRODA), Emene, Enugu State, Nigeria
b.
Department of Agricultural and Bioresource Engineering, Enugu State University of Science and Technology(ESUT), Enugu State, Nigeria

More Information

Corresponding author: Ude, Michael U., E-mail address:mikeudeproda@gmail.com
Received Date: 2020-05-10
Accepted Date: 2020-07-21
Rev Recd Date: 2020-06-30

Available Online: 2020-10-09

Publish Date: 2020-10-01

Abstract

Abstract

The optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels were carried out. The substrate was characterized using proximate analysis and hydrolysis. The process was optimized using response surface methodology while the kinetics of the hydrolysis was studied using Michealis-menten model. The results obtained showed that the mixed peels have high hemicellulose content, hydrolysable carbohydrate and cellulose. The optimum conditions for glucose yield of 79% by enzymatic hydrolysis are temperature of 36℃, time of five days, pH of 4.55 and enzyme dosage of 0.428 g/50mL. Enzymatic hydrolysis obeyed Michealis-Menten kinetic model with spontaneous and feasible reactions. Therefore, the results have proved that mixed peels (cassava and potato peels) is a good substrate for glucose production and study of other waste peels as feed-stock is recommended.
- enzymatic hydrolysis,
- optimization,
- glucose,
- cassava and potato peels

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References(14)

References

Adeeyo, O.A., Ayeni, A.O., Oladimeji, T.E., Oresegun, O.M., 2015. Acid hydrolysis of lignocellulosic content of sawdust to fermentable sugars for ethanol production. International Journal of Scientific and Engineering Research, 6, 890-899. http://www.ijser.org/paper/Acid-Hydrolysis-of-Lignocellulosic-Content-of-Sawdust-to-Fermentable-Sugars-for-Ethanol-Production.html

Ajani, A.O., Agarry, S.E., Agbede, O.O., 2011. A comparative kinetic study of acidic hydrolysis of wastes cellulose from agricultural derived biomass. Journal of Science and Environmental Management 15, 531-537. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ea6b67fa-4085-4538-b023-c2958402def4

Ana, D., Julie, le T., Ana, B., Ignacio, de O., Iidefonso, C., 2013. Pre-treatment of rice hulls with alkaline peroxide to enhance enzyme hydrolysis for ethanol production. Chemical Engineering Transactions 32, 949-954. http://www.researchgate.net/publication/273951995_Pretreatment_of_rice_hulls_with_alkaline_peroxide_to_enhance_enzyme_hydrolysis_for_ethanol_production

Badgujar, K.C., Bhanage, B.M., 2015. Thermo-chemical energy assessment for production of energy-rich fuel additive compounds by using levulinic acid and immobilized lipase. Fuel Process. Technol. 138, 139-146. doi: 10.1016/j.fuproc.2015.05.015

Fogler, H.S., 1999. Elements of chemical reaction engineering. New Delhi:Prentice Hall of India.

Hirakawa, H., Kamiya, N., Kawarabayashi, Y., Nagamune, T., 2005. Log P effect of organic solvents on a thermophilic alcohol dehydrogenase. Biochim. et Biophys. Acta BBA-Proteins Proteom. 1748, 94-99. doi: 10.1016/j.bbapap.2004.12.007

Jadhav, S.H., Gogate, P.R., 2014. Intensification in the activity of lipase enzyme using ultrasonic irradiation and stability studies. Ind. Eng. Chem. Res. 53, 1377-1385. doi: 10.1021/ie403419e

Kongkiattikajorn, J., 2012. Ethanol production from dilute acid pretreated cassava peel by fed-batch simultaneous saccharification and fermentation. International Journal of the Computer, the Internet and Management 20, 22-27.

Lalitha, G., Sivaraj, R., 2011. Use of Fruit Biomass Peel for Ethanol Production. International Journal of Pharmacy and Bio Sciences 2, 15-23. http://www.cabdirect.org/abstracts/20113339791.html

Manikandan, K., Sarananan, V., Viruthajru, T., 2008. Kinetics studies on ethanol production from banana peel waste using mutant strain of saccharomyces cerevisiae. Indian Journal of Biotechnology 7, 83-88. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=Open J-Gate000001348041

Omemu, A.M., Akpan, I., Bankole, M.O., Teniola, O.D., 2005. Hydrolysis of raw tuber starches by amylase of aspergillus niger AMO7 isolated from the soil. African J. of Biotechnol. 4, 19-25. http://www.cabdirect.org/abstracts/20053150472.html

Onyelucheya, Sarananan, V., Viruthajru, T., 2016. Kinetics studies on ethanol production from banana peel waste using mutant strain of saccharomyces cerevisiae. Indian J. Biotechnol. 7, 83-88. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=Open J-Gate000001348041

Pramanik, K., Rao, D.E., 2015. Kinetic study on ethanol fermentation of grape waste using Saccharomyces cerevisiae yeast isolated from toddy. Journal of Institution of Engineers (I) 85, 53-58. http://www.researchgate.net/publication/238775350_Kinetic_Study_on_Ethanol_Fermentation_of_Grape_Waste_using_Saccharomyces_cerevisiae_Yeast_Isolated_from_Toddy

Sun, Y., Cheng, J., 2002. Hydrolysis of lignocellulosic materials for ethanol production:a review. Bioresource Technol. 83, 1-11. doi: 10.1016/S0960-8524(01)00212-7

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