Improvement of quality of <i>Ginkgo biloba</i> seeds powder by solid-state fermentation with <i>Eurotium cristatum</i> for developing high-value ginkgo seeds products

Minmin Zou; Jiarui Cao; Wen Zhang; Chao Tang; Fuliang Cao; Erzheng Su

doi:10.1016/j.jobab.2021.10.002

Volume 7 Issue 2

May 2022

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Article Navigation > Journal of Bioresources and Bioproducts > 2022 > 7(2): 135-144

Minmin Zou, Jiarui Cao, Wen Zhang, Chao Tang, Fuliang Cao, Erzheng Su. Improvement of quality of Ginkgo biloba seeds powder by solid-state fermentation with Eurotium cristatum for developing high-value ginkgo seeds products[J]. Journal of Bioresources and Bioproducts, 2022, 7(2): 135-144. doi: 10.1016/j.jobab.2021.10.002

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Minmin Zou, Jiarui Cao, Wen Zhang, Chao Tang, Fuliang Cao, Erzheng Su. Improvement of quality of Ginkgo biloba seeds powder by solid-state fermentation with Eurotium cristatum for developing high-value ginkgo seeds products[J]. Journal of Bioresources and Bioproducts, 2022, 7(2): 135-144. doi: 10.1016/j.jobab.2021.10.002

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Improvement of quality of Ginkgo biloba seeds powder by solid-state fermentation with Eurotium cristatum for developing high-value ginkgo seeds products

doi: 10.1016/j.jobab.2021.10.002

Minmin Zou^{a, b},
Jiarui Cao^{a, b},
Wen Zhang^{a, b},
Chao Tang^a,
Fuliang Cao^b,
Erzheng Su^{a, b, c
,
,}

a.
Department of Food Science and Technology, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China
b.
Co-innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
c.
Co-Innovation Center for Efficient Processing and Utilization of Forest Products, Nanjing Forestry University, Nanjing 210037, China

More Information

Corresponding author: E-mail address: ezhsu@njfu.edu.cn (E. Su)
Received Date: 2021-04-11
Accepted Date: 2021-06-27
Rev Recd Date: 2021-06-20

Available Online: 2022-05-06

Publish Date: 2022-05-01

Abstract

Abstract

In this study, we investigated the feasibility of Ginkgo biloba seeds powder by solid-state fermentation with Eurotium cristatum for developing high-value ginkgo seeds products. The optimum fermentation medium was consisted of 10 g of 40-mesh ginkgo seeds powder loaded in 100 mL Erlenmeyer flask with 50% (w/w) of water content, 4% (w/w) of MgSO₄ and 5% (w/w) of KH₂PO₄ addition. The optimum fermentation conditions were pH 5.0, 2 × 10⁸ CFU/g of inoculum size, 3 mL of sterilized water supplemented every two days during the four days of fermentation. Through fermentation, the spore number of E. cristatum was improved by about 36 times with the production of lovastatin reaching (54.10±0.16) μg/g. The antioxidant activity of fermented ginkgo seeds powder also got obvious enhancement, which could help eliminate excess free radicals produced by normal metabolism. The content of free amino acids increased by 82.32%. Except that the sugar was consumed in some degree, the other nutritional and functional components were well preserved while the content of detrimental ginkgolic acids was reduced by 44.97%. In addition, fermented ginkgo seeds powder possessed better digestibility and showed pleasant orange-like smelling. In conclusion, the quality of ginkgo seeds powder was remarkably improved through solid-state fermentation by using E. cristatum, which could be a promising way for functional applications of ginkgo seeds.
- Ginkgo seed,
- Eurotium cristatum,
- Solid-state fermentation,
- Lovastatin,
- Antioxidant activity,
- Ginkgolic acid

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References(44)

References

Azeem, M., Saleem, Y., Hussain, Z., Zahoor, S., Javed, M.M., 2018. Optimization of culture conditions for the production of lovastatin by Aspergillus terreus in submerged fermentation. Pharm. Chem. J. 52, 284-289. doi: 10.1007/s11094-018-1807-4

Bosnjak, M., Mihaljevic, K., 2007. Continuous calorimetry, baker's yeast metabolism, anabolism, catabolism. Chem. Biochem. Eng. Q. 21, 409-416.

Cao, J., Chen, L.Y., Li, M.H., Cao, F.L., Zhao, L.G., Su, E.Z., 2018. Two-phase systems developed with hydrophilic and hydrophobic deep eutectic solvents for simulta-neously extracting various bioactive compounds with different polarities. Green Chem 20, 1879-1886. doi: 10.1039/C7GC03820H

Chan, P.C., Xia, Q.S., Fu, P.P., 2007. Ginkgo biloba leave extract: biological, medicinal, and toxicological effects. J. Environ. Sci. Heal. Part C 25, 211-244. doi: 10.1080/10590500701569414

Deng, F.M., Gong, S.L., Yang, W.L., 2007. Study on bioactivity of extracellular polysaccharide of Eurotium cristatum by high throughput screening. Food Mach 23, 113 48-51.

Du, F.Y., Li, X.M., Li, C.S., Shang, Z., Wang, B.G., 2012. Cristatumins A-D, new indole alkaloids from the marine-derived endophytic fungus Eurotium cristatum EN-220. Bioorg. Med. Chem. Lett. 22, 4650-4653. doi: 10.1016/j.bmcl.2012.05.088

Gong, H., Wu, C.E., Fan, G.J., Li, T.T., Wang, J.H., Wang, T., 2020. Determination of native contents of 4'-O-methylpyridoxine and its glucoside in raw and heated Ginkgo biloba seeds by high-performance liquid chromatography. J. Food Meas. Charact. 14, 917-924. doi: 10.1007/s11694-019-00341-y

Guo, N., Song, X.R., Kou, P., Zang, Y.P., Jiao, J., Efferth, T., Liu, Z.M., Fu, Y.J., 2018. Optimization of fermentation parameters with magnetically immobilized Bacillus natto on Ginkgo seeds and evaluation of bioactivity and safety. LWT 97, 172-179. doi: 10.1016/j.lwt.2018.06.046

Huang, Q., Chen, L.J., Li, Y.P., Che, K., 2007. Study on the effect of dark tea fermentation liquid with Eurotium cristatum on the activity of digestive enzyme. Microbiology 34, 917-920.

Huang, Y., Shi, R., Su, E.Z., 2017. Progress in the research and application of the Eurotium Cristatum. Chin. J. Bioprocess Eng. 15, 49-56.

Jiang, C.K., Zeng, Z., Huang, Y.H., Zhang, X., 2018. Chemical compositions of Pu'er tea fermented by Eurotium Cristatum and their lipid-lowering activity. LWT-Food Sci. Technol. 98, 204-211. doi: 10.1016/j.lwt.2018.08.007

Kajiyama, Y., Fujii, K., Takeuchi, H., Manabe, Y., 2002. Ginkgo seed poisoning. Pediatrics 109, 325-327. doi: 10.1542/peds.109.2.325

Kapoor, M., Nair, L.M., Kuhad, R.C., 2008. Cost-effective xylanase production from free and immobilized Bacillus pumilus strain MK001 and its application in saccha-rification of Prosopis juliflora. Biochem. Eng. J. 38, 88-97. doi: 10.1016/j.bej.2007.06.009

Li, Q., Sun, W., Jiang, Y., Cao, F.L., Wang, G.B., Zhao, L.G., 2017. Extraction and biodegradation of ginkgolic acidsfrom Ginkgo biloba sarcotestae. Front. Agr. Sci. Eng. 4, 465. doi: 10.15302/J-FASE-2017181

Liu, S.X., Liu, S.C., Li, Z.G., 2018. Effect of Eurotium cristatum on flavones content in Avena sativa L. China Brewing 38, 93-97.

Liu, Z.Y., Qin, J., 1991. Study on growth conditions of jinhua fungus in Fu zhuan tea. Guizhou Sci 9, 20-24.

Ma, J., Zhang, J., Sun, G.X., Lou, Y.N., Li, Y., 2019. Effects of dietary reduced glutathione on the growth and antioxidant capacity of juvenile Atlantic salmon (Salmo salar). Aquac. Nutr. 25, 1028-1035. doi: 10.1111/anu.12920

Molaverdi, M., Karimi, K., Khanahmadi, M., Goshadrou, A., 2013. Enhanced sweet Sorghum stalk to ethanol by fungus Mucor indicus using solid state fermentation followed by simultaneous saccharification and fermentation. Ind. Crop. Prod. 49, 580-585. doi: 10.1016/j.indcrop.2013.06.024

National Standard of the People's Republic of China, 2009. Determination of total sugar content in edible fungi (GB/T15672-2009). China Standard Press, Beijing.

National Standard of the People's Republic of China, 2014. Standard for the use of food additives (GB 2760-2014). China Standard Press, Beijing.

National Standard of the People's Republic of China, 2016a. Determination of oil in food (GB 5009.6-2016). China Standard Press, Beijing.

National Standard of the People's Republic of China, 2016b. Determination of reducing sugar in food (GB 5009.7-2016). China Standard Press, Beijing.

National Standard of the People's Republic of China, 2016c. Determination of starch in food (GB 5009.9-2016). China Standard Press, Beijing.

Parvez, S., Malik, K.A., Ah Kang, S., Kim, H.Y., 2006. Probiotics and their fermented food products are beneficial for health. J. Appl. Microbiol. 100, 1171-1185. doi: 10.1111/j.1365-2672.2006.02963.x

Ruiz, H.A., Rodríguez-Jasso, R.M., Rodríguez, R., Contreras-Esquivel, J.C., Aguilar, C.N., 2012. Pectinase production from lemon peel pomace as support and carbon source in solid-state fermentation column-tray bioreactor. Biochem. Eng. J. 65, 90-95. doi: 10.1016/j.bej.2012.03.007

Sandhya, C., Sumantha, A., Szakacs, G., Pandey, A., 2005. Comparative evaluation of neutral protease production by Aspergillus oryzae in submerged and solid-state fermentation. Process. Biochem. 40, 2689-2694. doi: 10.1016/j.procbio.2004.12.001

Singh, B., Kaur, P., Singh, R.D., Ahuja, P.S., 2008. Biology and chemistry of Ginkgo biloba. Fitoterapia 79, 401-418. doi: 10.1016/j.fitote.2008.05.007

Su, E.Z., Yang, M., Cao, J., Lu, C., Wang, J.H., Cao, F.L., 2017. Deep eutectic solvents as green media for efficient extraction of terpene trilactones from Ginkgo biloba leaves. J. Liq. Chromatogr. Relat. Technol. 40, 385-391. doi: 10.1080/10826076.2017.1309661

van Beek, T.A., Montoro, P., 2009. Chemical analysis and quality control of Ginkgo biloba leaves, extracts, and phytopharmaceuticals. J. Chromatogr. A 1216, 2002-2032. doi: 10.1016/j.chroma.2009.01.013

Wang, H.Y., Zhang, Y.Q., 2019. The main active constituents and detoxification process of Ginkgo biloba seeds and their potential use in functional health foods. J. Food Compos. Anal. 83, 103247. doi: 10.1016/j.jfca.2019.103247

Wang, J.H., Cao, F.L., Su, E.Z., Zhao, L.G., Qin, W.S., 2018a. Improvement of animal feed additives of Ginkgo leaves through solid-state fermentation using Aspergillus niger. Int. J. Biol. Sci. 14, 736-747. doi: 10.7150/ijbs.24523

Wang, J.H., Cao, F.L., Zhu, Z.L., Zhang, X.H., Sheng, Q.Q., Qin, W.S., 2018b. Improvement of quality and digestibility of Moringa oleifera leaves feed via solid-state fermentation by Aspergillus niger. Int. J. Chem. React. Eng. 16, 20180094.

Wang, Y.C., Tao, Y., Zhang, X.Y., Shao, S.J., Han, Y.B., Chu, D.T., Xie, G.J., Ye, X.S., 2019. Metabolic profile of Ginkgo kernel juice fermented with lactic aicd bacteria: a potential way to degrade ginkgolic acids and enrich terpene lactones and phenolics. Process. Biochem. 76, 25-33. doi: 10.1016/j.procbio.2018.11.006

Xu, A.Q., Wang, Y.L., Wen, J.Y., Liu, P., Liu, Z.Y., Li, Z.J., 2011. Fungal community associated with fermentation and storage of Fuzhuan brick-tea. Int. J. Food Microbiol. 146, 14-22. doi: 10.1016/j.ijfoodmicro.2011.01.024

Xun, J.Q., Luo, M.L., Zhang, X., Lin, F.Q., 2018. Research process on medical value of Eurotium cristatum. Sci. Technol. Food Ind. 39, 336-339.

Yang, J.M., Huang, J., Yang, M.H., 2015. Effects of the fermentation of jinghua fungus on the main chemical constituents of Tie-Guan-Yin tea. Guangdong Agr. Sci. 42, 20-26.

Zhang, B., Li, W., Dong, M.S., 2017. Flavonoids of kudzu root fermented by eurtotium cristatum protected rat pheochromocytoma line 12 (PC12) cells against H₂O₂-induced apoptosis. Int. J. Mol. Sci. 18, 2754. doi: 10.3390/ijms18122754

Zhang, B.B., Xing, H.B., Jiang, B.J., Chen, L., Xu, G.R., Jiang, Y., Zhang, D. Y, 2018. Using millet as substrate for efficient production of monacolin K by solid-state fermentation of Monascus ruber. J. Biosci. Bioeng. 125, 333-338. doi: 10.1016/j.jbiosc.2017.10.011

Zhang, H., Wang, Z., Xu, S.Y., 2007. Optimization of processing parameters for cloudy Ginkgo (Ginkgo biloba Linn. ) juice. J. Food Eng. 80, 1226-1232. doi: 10.1016/j.jfoodeng.2006.09.021

Zhang, W., Zou, M.M., Wu, R., Jiang, H.J., Cao, F.L., Su, E.Z., 2019. Insight into the transformation of 4'-O-methylpyridoxine and 4'-O-methylpyridoxine-5'-glucoside in Ginkgo biloba seeds undergoing the heat treatment. Ind. Crops Prod. 140, 111622. doi: 10.1016/j.indcrop.2019.111622

Zhou, H., Wang, C.Z., Ye, J.Z., Chen, H.X., Tao, R., Zhang, Y.S., 2015. Solid-state fermentation of Ginkgo biloba L. residue for optimal production of cellulase, protease and the simultaneous detoxification of Ginkgo biloba L. residue using Candida tropicalis and Aspergillus oryzae. Eur. Food Res. Technol. 240, 379-388. doi: 10.1007/s00217-014-2337-2

Zhou, S.D., Xu, X., Lin, Y.F., Xia, H.Y., Huang, L., Dong, M.S., 2019. On-line screening and identification of free radical scavenging compounds in Angelica dahurica fermented with Eurotium cristatum using an HPLC-PDA-Triple-TOF-MS/MS-ABTS system. Food Chem 272, 670-678. doi: 10.1016/j.foodchem.2018.07.173

Zou, M.M., Dong, Q.H., Huang, Y., Su, E.Z., 2018. Submerged liquid fermentation of raw dark tea by Eurotium cristatum. Chin. J. Bioprocess Eng. 17, 409-417.

Zou, M.M., Guo, X.H., Huang, Y., Cao, F.L., Su, E.Z., Wang, J.H., 2019. Improvement of the quality of Ginkgo biloba leaves fermented by Eurotium cristatum as high value-added feed. Processes 7, 627. doi: 10.3390/pr7090627

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