Exploitation of the antibacterial, antibiofilm and antioxidant activities of <i>Salvadora Persica</i> (Miswak) extract

M. El-Sherbiny Gamal; Mohamed Gazelly Ahmed; H. Sharaf Mohammed; A. Moghannemm Saad; M.E Shehata; K.A. Ismail Mahmoud; S. El-Hawary Ahmad

doi:10.1016/j.jobab.2022.11.006

Volume 8 Issue 1

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Article Navigation > Journal of Bioresources and Bioproducts > 2023 > 8(1): 59-65

M. El-Sherbiny Gamal, Mohamed Gazelly Ahmed, H. Sharaf Mohammed, A. Moghannemm Saad, M.E Shehata, K.A. Ismail Mahmoud, S. El-Hawary Ahmad. Exploitation of the antibacterial, antibiofilm and antioxidant activities of Salvadora Persica (Miswak) extract[J]. Journal of Bioresources and Bioproducts, 2023, 8(1): 59-65. doi: 10.1016/j.jobab.2022.11.006

Citation:

M. El-Sherbiny Gamal, Mohamed Gazelly Ahmed, H. Sharaf Mohammed, A. Moghannemm Saad, M.E Shehata, K.A. Ismail Mahmoud, S. El-Hawary Ahmad. Exploitation of the antibacterial, antibiofilm and antioxidant activities of Salvadora Persica (Miswak) extract[J]. Journal of Bioresources and Bioproducts, 2023, 8(1): 59-65. doi: 10.1016/j.jobab.2022.11.006

Citation:

M. El-Sherbiny Gamal, Mohamed Gazelly Ahmed, H. Sharaf Mohammed, A. Moghannemm Saad, M.E Shehata, K.A. Ismail Mahmoud, S. El-Hawary Ahmad. Exploitation of the antibacterial, antibiofilm and antioxidant activities of Salvadora Persica (Miswak) extract[J]. Journal of Bioresources and Bioproducts, 2023, 8(1): 59-65. doi: 10.1016/j.jobab.2022.11.006

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Exploitation of the antibacterial, antibiofilm and antioxidant activities of Salvadora Persica (Miswak) extract

doi: 10.1016/j.jobab.2022.11.006

Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo 11884, Egypt

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Corresponding author: E-mail address: gamalelsherbiny1970@azhar.edu.eg (G. El-Sherbiny)
Received Date: 2022-09-02
Accepted Date: 2022-11-07
Rev Recd Date: 2022-10-29

Available Online: 2022-12-01

Publish Date: 2023-02-01

Abstract

Abstract

The Salvadora persica (S. persica) L. chewing stick, usually known as miswak, is still being employed as an oral hygiene agent for plaque and gingivitis prevention. This study aims to assess the antibacterial, antibiofilm, antioxidant, and phytochemical profile of S. persica extract. The S. persica was purchased from a local market, grinded and extracted with petroleum ether. The disk diffusion, microdilution, and micro-plate assays were performed to evaluate the antibacterial and antibiofilm activities of the prepared extract at different concentrations against β-lactam resistance Streptococcus species. Free radical scavenging 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and stable radical cationic chromophore, 2, 2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) methods were used to determine their antioxidant activity. Chromatographic and spectrometric analyses were performed using gas chromatography-mass (GC-MS) spectrometry. The minimum inhibitory concentration (MIC) of S. persica extract against β-lactam resistance Streptococcus species ranged from 6.25 to 12.5 mg/mL. The maximum suppression of biofilm formation by S. persica extract was observed at MIC with a percentage of 68.66%, against Streptococcus oralis. The S. persica extract exhibited antioxidant activity with IC₅₀ of 20 µg/mL and 35 µg/mL from DPPH and ABTS, respectively. The phytochemical characterization showed the presence of 22 compounds with major compounds; benzyl isothiocyanate (36.21%) and n-hexadecanoic acid (27.62%). The S. persica extract exhibited antibacterial activity against β-lactam resistant Streptococcus species, showing a promising natural alternative that could be a treatment option.
- β-lactam resistance Streptococcus species,
- S. persica extract,
- Antibacterial,
- Antibiofilm,
- Antioxidant,
- GC-MS analysis

Conflict of Interest The author has no conflicts of interest that are concerned with this article.

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References(38)

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