Volume 8 Issue 4
Oct.  2023
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Article Navigation >  Journal of Bioresources and Bioproducts  > 2023  >  8(4): 461-477
Zhaoxian Xu, Jie Li, Pingping Li, Chenggu Cai, Sitong Chen, Boning Ding, Shuangmei Liu, Mianshen Ge, Mingjie Jin. Efficient lignin biodegradation triggered by alkali-tolerant ligninolytic bacteria through improving lignin solubility in alkaline solution[J]. Journal of Bioresources and Bioproducts, 2023, 8(4): 461-477. doi: 10.1016/j.jobab.2023.09.004
Citation: Zhaoxian Xu, Jie Li, Pingping Li, Chenggu Cai, Sitong Chen, Boning Ding, Shuangmei Liu, Mianshen Ge, Mingjie Jin. Efficient lignin biodegradation triggered by alkali-tolerant ligninolytic bacteria through improving lignin solubility in alkaline solution[J]. Journal of Bioresources and Bioproducts, 2023, 8(4): 461-477. doi: 10.1016/j.jobab.2023.09.004
shu

Efficient lignin biodegradation triggered by alkali-tolerant ligninolytic bacteria through improving lignin solubility in alkaline solution

doi: 10.1016/j.jobab.2023.09.004
  • a.

    School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China

  • b.

    Biorefinery Research Institution, Nanjing University of Science and Technology, Nanjing, 210094, China

More Information
  • Corresponding author: E-mail address: jinmingjie@njust.edu.cn (M. Jin)
  • Available Online: 2023-10-11
  • Publish Date: 2023-10-28
    Abstract
  • Low lignin solubility in aqueous solution is one of the major bottlenecks for lignin biodegradation and bioconversion. Alkaline solution contributes to improving lignin solubility, whereas most microbes can not survive in alkaline conditions. Herein, lignin dissolution behaviors in different pH solutions were systematically investigated, which indicated that solution pH above 10.5 contributed to high solubility of alkali lignin. To match with alkaline lignin aqueous system, several alkali-tolerant ligninolytic bacteria were isolated, most of which are distinct to previously reported ones. Then, the ligninolytic capabilities of these isolates were assessed in different pH conditions by determining their assimilation on alkali lignin, lignin-derived monomers and dimers, their decolorization capabilities, and their lignin peroxidase activities. Thereafter, the underlying ligninolytic and alkali-tolerant mechanisms of Sutcliffiella sp. NC1, an alkalophilic bacterium, was analyzed on the basis of its genome information. The results not only provide valuable information for lignin biodegradation and lignin valorization, but also expand knowledge on alkali-tolerant bacteria.

     

    • Declaration of Competing Interest
    The authors declare no conflict of interest.
    Supplementary materials
    Supplementary material associated with this article can be found, in the online version, at doi:10.1016/j.jobab.2023.09.004.
    1 These authors contributed equally to this work.
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