In situ biosynthesis of bacterial cellulose hydrogel spheroids with tunable dimensions

Bianjing Sun; Ping Wang; Jingang Zhang; Jianbin Lin; Lingling Sun; Xiaokun Wang; Chuntao Chen; Dongping Sun

doi:10.1016/j.jobab.2023.12.003

Volume 9 Issue 1

Feb. 2024

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Article Navigation > Journal of Bioresources and Bioproducts > 2024 > 9(1): 90-101

Bianjing Sun, Ping Wang, Jingang Zhang, Jianbin Lin, Lingling Sun, Xiaokun Wang, Chuntao Chen, Dongping Sun. In situ biosynthesis of bacterial cellulose hydrogel spheroids with tunable dimensions[J]. Journal of Bioresources and Bioproducts, 2024, 9(1): 90-101. doi: 10.1016/j.jobab.2023.12.003

Citation:

Bianjing Sun, Ping Wang, Jingang Zhang, Jianbin Lin, Lingling Sun, Xiaokun Wang, Chuntao Chen, Dongping Sun. In situ biosynthesis of bacterial cellulose hydrogel spheroids with tunable dimensions[J]. Journal of Bioresources and Bioproducts, 2024, 9(1): 90-101. doi: 10.1016/j.jobab.2023.12.003

Citation:

Bianjing Sun, Ping Wang, Jingang Zhang, Jianbin Lin, Lingling Sun, Xiaokun Wang, Chuntao Chen, Dongping Sun. In situ biosynthesis of bacterial cellulose hydrogel spheroids with tunable dimensions[J]. Journal of Bioresources and Bioproducts, 2024, 9(1): 90-101. doi: 10.1016/j.jobab.2023.12.003

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In situ biosynthesis of bacterial cellulose hydrogel spheroids with tunable dimensions

doi: 10.1016/j.jobab.2023.12.003

Bianjing Sun^{a, 1},
Ping Wang^{a, 1},
Jingang Zhang^{b, 1},
Jianbin Lin^a,
Lingling Sun^a,
Xiaokun Wang^a,
Chuntao Chen^{a
,
,},
Dongping Sun^{a
,
,}

a.
Institute of Chemicobiology and Functional Materials, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China
b.
Zhonghao Chenguang Chemical Research Institute Co., Ltd., Zigong 643201, China

More Information

Corresponding author: E-mail address: chchunt@njust.edu.cn (C. Chen); E-mail address: hysdp@njust.edu.cn (D. Sun)
Available Online: 2023-12-26
Publish Date: 2024-02-01

Abstract

Abstract

Bacterial cellulose (BC) hydrogel spheroid plays a significant role in diverse fields due to its spatial 3D structure and properties. In the present work, a series of BC spheroids with controllable size and shape was obtained via an in situ biosynthesis. Crucial factors for fabricating BC spheroid including inoculum concentration of 1.35 × 10³ CFU/mL, shaking speeds at 100 r/min, and 48–96 h incubation time during the biosynthetic process, were comprehensively established. An operable mechanism model for tuning the size of BC spheroids from 0.4 to 5.0 mm was proposed with a fresh feeding medium strategy of dynamic culture. The resulting BC spheroids exhibit an interactive 3D network of nanofibers, a crystallinity index of 72.3 %, a specific surface area of 91.2 m²/g, and good cytocompatibility. This study reinforces the understanding of BC spheroid formation and explores new horizons for the design of BC spheroids-derived functional matrix materials for medical care.
- In situ biosynthesis,
- Bacterial cellulose,
- Hydrogel,
- Spheroid,
- Tunable dimension

Data availability
Data will be made available on request.
Declarations of Competing Interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Supplementary materials
Supplementary material associated with this article can be found, in the online version, at doi:10.1016/j.jobab.2023.12.003
¹ These authors contributed equally to this work.

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References(38)

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