From common biomass materials to high-performance tissue engineering scaffold: Biomimetic preparation, properties characterization, <i>in vitro</i> and <i>in vivo</i> evaluations

Zongpu Xu; Fang He; Jing Yu; Zhangze Yang; Yu Zhu; Rong Liao; Ruyin Lyu; Mei Yang; Liangjun Zhu; Mingying Yang

doi:10.1016/j.jobab.2024.03.004

Volume 9 Issue 2

May 2024

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Article Navigation > Journal of Bioresources and Bioproducts > 2024 > 9(2): 185-196

Zongpu Xu, Fang He, Jing Yu, Zhangze Yang, Yu Zhu, Rong Liao, Ruyin Lyu, Mei Yang, Liangjun Zhu, Mingying Yang. From common biomass materials to high-performance tissue engineering scaffold: Biomimetic preparation, properties characterization, in vitro and in vivo evaluations[J]. Journal of Bioresources and Bioproducts, 2024, 9(2): 185-196. doi: 10.1016/j.jobab.2024.03.004

Citation:

Zongpu Xu, Fang He, Jing Yu, Zhangze Yang, Yu Zhu, Rong Liao, Ruyin Lyu, Mei Yang, Liangjun Zhu, Mingying Yang. From common biomass materials to high-performance tissue engineering scaffold: Biomimetic preparation, properties characterization, in vitro and in vivo evaluations[J]. Journal of Bioresources and Bioproducts, 2024, 9(2): 185-196. doi: 10.1016/j.jobab.2024.03.004

Citation:

Zongpu Xu, Fang He, Jing Yu, Zhangze Yang, Yu Zhu, Rong Liao, Ruyin Lyu, Mei Yang, Liangjun Zhu, Mingying Yang. From common biomass materials to high-performance tissue engineering scaffold: Biomimetic preparation, properties characterization, in vitro and in vivo evaluations[J]. Journal of Bioresources and Bioproducts, 2024, 9(2): 185-196. doi: 10.1016/j.jobab.2024.03.004

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From common biomass materials to high-performance tissue engineering scaffold: Biomimetic preparation, properties characterization, in vitro and in vivo evaluations

doi: 10.1016/j.jobab.2024.03.004

a. Institute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China;
b. Key Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, Zhejiang University, Hangzhou 310058, China;
c. Department of Neurosurgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China

Funds:

This work was supported by National Natural Science Foundation of China (No. 52103149), State of Sericulture Industry Technology System (No. CARS-18-ZJ0501), Key Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province (No. 2020E10025), Zhejiang University start-up fund, and the program “Construction of Mineralized Silk Fibroin Microfiber Reinforced Chitosan Composite Scaffold and its Application in Bone Repair”. Zongpu Xu thanks the undergraduates Guicong Zhang, Zhen He, and Mingzheng Fang from Zhejiang University for their help with experiments.

Publish Date: 2024-03-26

Abstract

Abstract

Converting common biomass materials to high-performance biomedical products could not only reduce the environmental pressure associated with the large-scale use of synthetic materials, but also increase the economic value. Chitosan as a very promising candidate has drawn considerable attention owing to its abundant sources and remarkable bioactivities. However, pure chitosan materials usually exhibit insufficient mechanical properties and excessive swelling ratio, which seriously affected their in vivo stability and integrity when applied as tissue engineering scaffolds. Thus, simultaneously improving the mechanical strength and biological compatibility of pure chitosan (CS) scaffolds becomes very important. Here, inspired by the fiber-reinforced construction of natural extracellular matrix and the porous structure of cancellous bone, we built silk microfibers/chitosan composite scaffolds via ice-templating technique. This biomimetic strategy achieved 500% of mechanical improvement to pure chitosan, and meanwhile still maintaining high porosity (> 87%). In addition, the increased roughness of chitosan pore walls by embedded silk microfibers significantly promoted cell adhesion and proliferation. More importantly, after subcutaneous implantation in mice for four weeks, the composite scaffold showed greater structural integrity, as well as better collagenation, angiogenesis, and osteogenesis abilities, suggesting its great potential in biomedicine.
- Chitosan,
- Biomimetic strategy,
- Fiber-reinforced composite,
- Mechanical property,
- Biocompatibility

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References(69)

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