Volume 9 Issue 3
Aug.  2024
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Article Navigation >  Journal of Bioresources and Bioproducts  > 2024  >  9(3): 336-350
Xucai Wang, Dengxian Wu, Wei Liao, Yaxuan Liu, Wenhui Pei, Jixian Wang, Jiayu Gu, Peng Wang, Kai Lan, Caoxing Huang. Constructing osteo-inductive bio-ink for 3D printing through hybridization of gelatin with maleic acid modified bacterial cellulose by regulating addition volumes of maleic acid solution[J]. Journal of Bioresources and Bioproducts, 2024, 9(3): 336-350. doi: 10.1016/j.jobab.2024.04.001
Citation: Xucai Wang, Dengxian Wu, Wei Liao, Yaxuan Liu, Wenhui Pei, Jixian Wang, Jiayu Gu, Peng Wang, Kai Lan, Caoxing Huang. Constructing osteo-inductive bio-ink for 3D printing through hybridization of gelatin with maleic acid modified bacterial cellulose by regulating addition volumes of maleic acid solution[J]. Journal of Bioresources and Bioproducts, 2024, 9(3): 336-350. doi: 10.1016/j.jobab.2024.04.001
shu

Constructing osteo-inductive bio-ink for 3D printing through hybridization of gelatin with maleic acid modified bacterial cellulose by regulating addition volumes of maleic acid solution

doi: 10.1016/j.jobab.2024.04.001
  • a.

    Co-Innovation Center for Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China

  • b.

    State Key Laboratory of Pharmaceutical Biotechnology, Department of Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nahanjing 210008, China

  • c.

    Children’s Hospital of Nanjing Medical University, Nanjing 210008, China

  • d.

    Jiangsu Institute of Metrology, Nanjing 210023, China

  • e.

    Department of Forest Biomaterials, College of Natural Resources, North Carolina State University, Raleigh NC 27695, USA

More Information
  • Corresponding author: E-mail address: hcx@njfu.edu.cn (C. Huang)
  • Available Online: 2024-04-04
  • Publish Date: 2024-07-05
    Abstract
  • Bacterial cellulose (BC) is an exopolysaccharide with unique properties that has been applied in various fields. However, the dense and intertwined nature of BC fibers limits its use in certain applications, including 3D printing scaffolds for bone regeneration. In this work, a controllable BC-based bio-ink for 3D printing was successfully prepared by modifying the neat BC through maleic acid (MA) treatment, aiming to promote bone tissue regeneration. To achieve homogeneous BC dispersions while preserving its crystalline and chemical properties, BC was modified by MA solution (60 %, w/V) with solid-liquid ratio from 1꞉5 to 1꞉50 (w/V) to obtain MA-BC dispersions. The analysis results from microstructure, chemical group, crystallinity, and wettability indicated that the BC/MA solution with ratio of 1꞉30 demonstrated the best pre-treatment performance to obtain MA-BC. Subsequently, by combining MA-BC with gelatin, we successfully formulated MA-BC-GEL gels with favorable rheological properties and compression modulus, which can be used as promising bio-inks for 3D bioprinting applications. In vitro tests demonstrated 1꞉30 MA-BC possessed excellent biocompatibility, a significant ability to express the alkaline phosphatase gene and osteogenic-related genes, and facilitated the formation of mineralized nodules. The utilization of this novel bio-ink in scaffold preparation for bone regeneration highlights the promising application of modified BC in bone tissue engineering field.

     

    • Declaration of competing interest
    The authors declare no conflict of interest.
    CRediT authorship contribution statement
    Xucai Wang: Formal analysis, Investigation, Writing – original draft. Dengxian Wu: Investigation, Writing – original draft. Wei Liao: Writing – original draft. Yaxuan Liu: Methodology. Wenhui Pei: Formal analysis, Methodology. Jixian Wang: Methodology. Jiayu Gu: Investigation. Peng Wang: Conceptualization, Writing – review & editing. Kai Lan: Conceptualization. Caoxing Huang: Conceptualization, Writing – review & editing.
    Ethics approval and consent to participate
    Not applicable.
    Consent for publication
    Not applicable.
    Availability of data
    Data available on request from the authors.
    Supplementary materials
    Supplementary material associated with this article can be found, in the online version, at doi:10.1016/j.jobab.2024.04.001.
    1 The authors contributed equally to this work and should be considered co-first authors.
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