Volume 10 Issue 2
May  2025
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Article Navigation >  Journal of Bioresources and Bioproducts  > 2025  >  10(2): 224-238
Quan Zhou, Zijing Zhao, Litao Wang, Jiandong Wang, Lina Fu, Jihong Cui, Guosheng Liu, Jie Yang, Yujie Fu. Immobilized enzyme microreactor system with bamboo-based cellulose nanofibers for efficient biotransformation of phytochemicals[J]. Journal of Bioresources and Bioproducts, 2025, 10(2): 224-238. doi: 10.1016/j.jobab.2025.03.004
Citation: Quan Zhou, Zijing Zhao, Litao Wang, Jiandong Wang, Lina Fu, Jihong Cui, Guosheng Liu, Jie Yang, Yujie Fu. Immobilized enzyme microreactor system with bamboo-based cellulose nanofibers for efficient biotransformation of phytochemicals[J]. Journal of Bioresources and Bioproducts, 2025, 10(2): 224-238. doi: 10.1016/j.jobab.2025.03.004
shu

Immobilized enzyme microreactor system with bamboo-based cellulose nanofibers for efficient biotransformation of phytochemicals

doi: 10.1016/j.jobab.2025.03.004
  • a.

    State Key Laboratory of Efficient Production of Forest Resources, Beijing Forestry University, Beijing 100083, China

  • b.

    The College of Forestry, Beijing Forestry University, Beijing 100083, China

  • c.

    The Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing 100083, China

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    Abstract
  • The enzyme immobilization technique has been widely applied in biotechnology, biomedicine, and environmental remediation. In this research, carboxylated bamboo-based cellulose nanofibers (BCNFs) were obtained by one-step oxidation modification of bamboo fibers using ammonium persulphate. The surface carboxyl groups of the BCNFs were modified by a crosslinking agent and then combined with polyethylene imine (PEI) functionalized magnetic nanoparticles to construct a microreactor system for enzyme loading by the methods of electrostatic self-assembly and physical adsorption. Contrasted with free β-glucosidase, the microreactor possesses higher relative enzyme activity at pH 5.5 and 50 ℃, and the storage stability is significantly higher, with >75% relative enzyme activity after storage at 4 ℃ for 15 d. In addition, the β-glucosidase loaded on the microreactor facilitates its separation from the reaction medium and subsequent reuse. After completing five cycles of use, it retained 76.47% of its initial activity. The biotransformation of geniposide reached 93.10%, and the genipin concentration increased 1.2 folds higher than that in the original plant extract. Therefore, PEI@Fe3O4@BCNFs microreactor immobilized with β-glucosidase can be successfully used to produce higher activity aglucone such as genipin from geniposide, and it might also have the potential to convert phytochemicals by the immobilized enzyme microreactor system with bamboo-based cellulose nanofibers in the natural production field.

     

    • The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
    Declaration of competing interest
    Quan Zhou: Designed the experiments, writing original draft, conceptualization, methodology, data curation; Zijing Zhao: Investigation, methodology; Litao Wang: Material characterizations, reviewed the manuscript; Jiandong Wang: Material characterizations; Lina Fu: Methodology for enzyme activity calculation; Jihong Cui: Methodology for enzyme activity calculation; Guosheng Liu: Methodology of high-performance liquid chromatography; Jie Yang and Yujie Fu: Designed the experiments, reviewed the manuscript, funding acquisition.
    CRediT authorship contribution statement
    Data availability
    Data will be made available on request.
    Supplementary material associated with this article can be found, in the online version, at doi:10.1016/j.jobab.2025.03.004.
    Supplementary materials
    1 These authors contributed equally to this work and should be considered co-first authors.
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