Volume 6 Issue 1
Feb.  2021
Turn off MathJax
Article Contents
Atiya Fatima, Yasir Sumayia, Mohd.Shariq Khan, Sehrish Manan, Muhammad Wajid Ullah, Mazhar Ul-Islam. Plant Extract-loaded Bacterial Cellulose Composite Membrane for Potential Biomedical Applications[J]. Journal of Bioresources and Bioproducts, 2021, 6(1): 26-32. doi: 10.1016/j.jobab.2020.11.002
Citation: Atiya Fatima, Yasir Sumayia, Mohd.Shariq Khan, Sehrish Manan, Muhammad Wajid Ullah, Mazhar Ul-Islam. Plant Extract-loaded Bacterial Cellulose Composite Membrane for Potential Biomedical Applications[J]. Journal of Bioresources and Bioproducts, 2021, 6(1): 26-32. doi: 10.1016/j.jobab.2020.11.002

Plant Extract-loaded Bacterial Cellulose Composite Membrane for Potential Biomedical Applications

doi: 10.1016/j.jobab.2020.11.002
More Information
  • Bacterial cellulose (BC) has been extensively explored as biomaterial for various biomedical applications owing to its non-toxic nature and unique structural morphology and impressive physico-chemical and mechanical properties. However, its high production cost and lack of antimicrobial activity have restricted its large-scale production and therapeutic applications. Therefore, the current study is aimed to devise a strategy for low-cost BC production and develop its composite with bioactive materials to bless it with antimicrobial activity. Herein, 5 mm thick reticulated fibrous and highly porous BC was produced by utilizing the wasted rotten tomatoes as the production medium. The produced bacterial cellulose waste (BCW) (i.e., produced from wastes) was ex-situ modified with bioactive plant extract (PE) obtained from Euclea schimperi, and the bactericidal activity of the developed BCW/PE was evaluated against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli through disc diffusion and colony forming unit (CFU) count methods. The BCW/PE composite showed high bactericidal activities against S. aureus and produced clear inhibition zone whereas negligible activity was observed against E. coli, indicating its bactericidal activity mainly against the Gram-positive bacterium. Overall, this study illustrates that there is a huge potential for developing valuable biomaterials from food wastes and utilizing their liquid holding capabilities for value-added applications in medical and pharmaceutical fields.


  • loading
  • Aljohani, W. , Ullah, M. W. , Zhang, X. L. , Yang, G. , 2018. Bioprinting and its applications in tissue engineering and regenerative medicine. Int. J. Biol. Macromol. 107, 261-275. doi: 10.1016/j.ijbiomac.2017.08.171
    Chen, X. L. , Huang, L. , Sun, H. J. , Cheng, S. Z. D. , Zhu, M. Q. , Yang, G. , 2014. Stimuli-responsive nanocomposite: potential injectable embolization agent. Macromol. Rapid Commun. 35, 579-584. doi: 10.1002/marc.201300720
    Di, Z. , Shi, Z. J. , Ullah, M. W. , Li, S. X. , Yang, G. , 2017. A transparent wound dressing based on bacterial cellulose whisker and poly(2-hydroxyethyl methacrylate). Int. J. Biol. Macromol. 105, 638-644. doi: 10.1016/j.ijbiomac.2017.07.075
    Gebre-Mariam, T. , Neubert, R. , Schmidt, P. C. , Wutzler, P. , Schmidtke, M. , 2006. Antiviral activities of some Ethiopian medicinal plants used for the treatment of dermatological disorders. J. Ethnopharmacol. 104, 182-187. doi: 10.1016/j.jep.2005.08.071
    Geisel, N. , Clasohm, J. , Shi, X. D. , Lamboni, L. , Yang, J. C. , Mattern, K. , Yang, G. , Schäfer, K. H. , Saumer, M. , 2016. Microstructured multilevel bacterial cellulose allows the guided growth of neural stem cells. Small 12, 5407-5413. doi: 10.1002/smll.201601679
    Giday, M. , Asfaw, Z. , Elmqvist, T. , Woldu, Z. , 2003. An ethnobotanical study of medicinal plants used by the Zay people in Ethiopia. J. Ethnopharmacol. 85, 43-52. doi: 10.1016/S0378-8741(02)00359-8
    Ibrar, M. , Ullah, M. W. , Manan, S. , Farooq, U. , Rafiq, M. , Hasan, F. , 2020. Fungi from the extremes of life: an untapped treasure for bioactive compounds. Appl. Microbiol. Biotechnol. 104, 2777-2801. doi: 10.1007/s00253-020-10399-0
    Islam, M. U. , Ullah, M. W. , Khan. S. , Shah, N. , Park, J. K. , 2017. Strategies for cost-effective and enhanced production of bacterial cellulose. Int. J. Biol. Macromol. 102, 1166-1173. doi: 10.1016/j.ijbiomac.2017.04.110
    Kamal, T. , Ahmad, I. , Khan, S. B. , Asiri, A. M. , 2019a. Anionic polysaccharide stabilized nickel nanoparticles-coated bacterial cellulose as a highly efficient dip-catalyst for pollutants reduction. React. Funct. Polym. 145, 104395. doi: 10.1016/j.reactfunctpolym.2019.104395
    Kamal, T. , Ahmad, I. , Khan, S. B. , Asiri, A. M. , 2019b. Bacterial cellulose as support for biopolymer stabilized catalytic cobalt nanoparticles. Int. J. Biol. Macromol. 135, 1162-1170. doi: 10.1016/j.ijbiomac.2019.05.057
    Kamal, T. , Ahmad, I. , Khan, S. B. , Ul-Islam, M. , Asiri, A. M. , 2019c. Microwave assisted synthesis and carboxymethyl cellulose stabilized copper nanoparticles on bacterial cellulose nanofibers support for pollutants degradation. J. Polym. Environ. 27, 2867-2877. doi: 10.1007/s10924-019-01565-1
    Khan, S. , Ul-Islam, M. , Ikram, M. , Ullah, M. W. , Israr, M. , Subhan, F. , Kim, Y. , Jang, J. H. , Yoon, S. , Park, J. K. , 2016. Three-dimensionally microporous and highly biocompatible bacterial cellulose-gelatin composite scaffolds for tissue engineering applications. RSC Adv. 6, 110840-110849. doi: 10.1039/C6RA18847H
    Khan, S. , Ul-Islam, M. , Khattak, W. A. , Ullah, M. W. , Park, J. K. , 2015a. Bacterial cellulose-poly(3, 4-ethylenedioxythiophene)-poly(styrenesulfonate) composites for optoelectronic applications. Carbohydr. Polym. 127, 86-93. doi: 10.1016/j.carbpol.2015.03.055
    Khan, S. , Ul-Islam, M. , Khattak, W. A. , Ullah, M. W. , Park, J. K. , 2015b. Bacterial cellulose-titanium dioxide nanocomposites: nanostructural characteristics, antibacterial mechanism, and biocompatibility. Cellulose 22, 565-579. doi: 10.1007/s10570-014-0528-4
    Kim, Y. , Ullah, M. W. , Ul-Islam, M. , Khan, S. , Jang, J. H. , Park, J. K. , 2019. Self-assembly of bio-cellulose nanofibrils through intermediate phase in a cell-free enzyme system. Biochem. Eng. J. 142, 135-144. doi: 10.1016/j.bej.2018.11.017
    Klemm, D. , Schumann, D. , Udhardt, U. , Marsch, S. , 2001. Bacterial synthesized cellulose: artificial blood vessels for microsurgery. Prog. Polym. Sci. 26, 1561-1603. doi: 10.1016/S0079-6700(01)00021-1
    Lamboni, L. , Xu, C. , Clasohm, J. , Yang, J. C. , Saumer, M. , Schäfer, K. H. , Yang, G. , 2019. Silk sericin-enhanced microstructured bacterial cellulose as tissue engineering scaffold towards prospective gut repair. Mater. Sci. Eng. : C 102, 502-510. doi: 10.1016/j.msec.2019.04.043
    Li, S. , Jasim, A. , Zhao, W. , 2018. Fabrication of pH-electroactive bacterial cellulose/polyaniline hydrogel for the development of a controlled drug release system. ES Mater. Manuf. 41-49.
    Li, S. H. , Huang, D. K. , Zhang, B. Y. , Xu, X. B. , Wang, M. K. , Yang G. , Shen, Y. , 2014. Flexible supercapacitors based on bacterial cellulose paper electrodes. Adv. Energy Mater. 4, 1301655. doi: 10.1002/aenm.201301655
    Liu, T. L. , Miao, J. C. , Sheng, W. H. , Xie, Y. F. , Huang, Q. , Shan, Y. B. , Yang, J. C. , 2010. Cytocompatibility of regenerated silk fibroin film: a medical biomaterial applicable to wound healing. J. Zhejiang Univ. Sci. B 11, 10-16. doi: 10.1631/jzus.B0900163
    McCarthy, R. R. , Ullah, M. W. , Booth, P. , Pei, E. , Yang, G. , 2019. The use of bacterial polysaccharides in bioprinting. Biotechnol. Adv. 37, 107448. doi: 10.1016/j.biotechadv.2019.107448
    Mekonnen, A. , Atlabachew, M. , Kassie, B. , 2018. Investigation of antioxidant and antimicrobial activities of Euclea schimperi leaf extracts. Chem. Biol. Technol. Agric. 5, 16. doi: 10.1186/s40538-018-0128-x
    Mogoşanu, G. D. , Grumezescu, A. M. , 2014. Natural and synthetic polymers for wounds and burns dressing. Int. J. Pharm. 463, 127-136. doi: 10.1016/j.ijpharm.2013.12.015
    Nwachukwu, C. U. , Umeh, C. N. , Kalu, I. G. , Okere S. , Nwoko, M. C. , 2010. Identification and traditional uses of some common medicinal plants in Ezinihitte Mbaise L. G. A. , of Imo State, Nigeria. Rep. Opin. 2, 1-8.
    Shah, N. , Ul-Islam, M. , Khattak, W. A. , Park, J. K. , 2013. Overview of bacterial cellulose composites: a multipurpose advanced material. Carbohydr. Polym. 98, 1585-1598. doi: 10.1016/j.carbpol.2013.08.018
    Shi, Z. J. , Zhang, Y. , Phillips, G. O. , Yang, G. , 2014. Utilization of bacterial cellulose in food. Food Hydrocoll. 35, 539-545. doi: 10.1016/j.foodhyd.2013.07.012
    Shoukat, A. , Wahid, F. , Khan, T. , 2019. Titanium oxide-bacterial cellulose bioadsorbent for the removal of lead ions from aqueous solution. Int. J. Biol. Macromol. 129, 965-971. doi: 10.1016/j.ijbiomac.2019.02.032
    Tang, W. H. , Jia, S. R. , Jia, Y. Y. , Yang, H. J. , 2010. The influence of fermentation conditions and post-treatment methods on porosity of bacterial cellulose membrane. World J. Microbiol. Biotechnol. 26, 125-131. doi: 10.1007/s11274-009-0151-y
    Ul-Islam, M. , Khan, S. , Ullah, M. W. , Park, J. K. , 2015. Bacterial cellulose composites: synthetic strategies and multiple applications in bio-medical and electro-conductive fields. Biotechnol. J. 10, 1847-1861. doi: 10.1002/biot.201500106
    Ul-Islam, M. , Khan, T. , Khattak, W. A. , Park, J. K. , 2013a. Bacterial cellulose-MMTs nanoreinforced composite films: novel wound dressing material with antibacterial properties. Cellulose 20, 589-596. doi: 10.1007/s10570-012-9849-3
    Ul-Islam, M. , Khan, T. , Park, J. K. , 2012. Water holding and release properties of bacterial cellulose obtained by in situ and ex situ modification. Carbohydr. Polym. 88, 596-603. doi: 10.1016/j.carbpol.2012.01.006
    Ul-Islam, M. , Khattak, W. A. , Kang, M. , Kim, S. M. , Khan, T. , Park, J. K. , 2013b. Effect of post-synthetic processing conditions on structural variations and applications of bacterial cellulose. Cellulose 20, 253-263. doi: 10.1007/s10570-012-9799-9
    Ul-Islam, M. , Khattak, W. A. , Ullah, M. W. , Khan, S. , Park, J. K. , 2014. Synthesis of regenerated bacterial cellulose-zinc oxide nanocomposite films for biomedical applications. Cellulose 21, 433-447. doi: 10.1007/s10570-013-0109-y
    Ul-Islam, M. , Subhan, F. , Islam, S. U. , Khan, S. , Shah, N. , Manan, S. , Ullah, M. W. , Yang, G. , 2019. Development of three-dimensional bacterial cellulose/chitosan scaffolds: Analysis of cell-scaffold interaction for potential application in the diagnosis of ovarian cancer. Int. J. Biol. Macromol. 137, 1050-1059. doi: 10.1016/j.ijbiomac.2019.07.050
    Ul-Islam, M. , Ullah, M. W. , Khan, S. , Park, J. K. , 2020. Production of bacterial cellulose from alternative cheap and waste resources: a step for cost reduction with positive environmental aspects. Korean J. Chem. Eng. 37, 925-937. doi: 10.1007/s11814-020-0524-3
    Ullah, M. W. , Ul Islam, M. , Khan, S. , Shah, N. , Park, J. K. , 2017. Recent advancements in bioreactions of cellular and cell-free systems: a study of bacterial cellulose as a model. Korean J. Chem. Eng. 34, 1591-1599. doi: 10.1007/s11814-017-0121-2
    Ullah, M. W. , Ul-Islam, M. , Khan, S. , Kim, Y. , Jang, J. H. , Park, J. K. , 2016a. In situ synthesis of a bio-cellulose/titanium dioxide nanocomposite by using a cell-free system. RSC Adv. 6, 22424-22435. doi: 10.1039/C5RA26704H
    Ullah, M. W. , Ul-Islam, M. , Khan, S. , Kim, Y. , Park, J. K. , 2015. Innovative production of bio-cellulose using a cell-free system derived from a single cell line. Carbohydr. Polym. 132, 286-294. doi: 10.1016/j.carbpol.2015.06.037
    Ullah, M. W. , Ul-Islam, M. , Khan, S. , Kim, Y. , Park, J. K. , 2016b. Structural and physico-mechanical characterization of bio-cellulose produced by a cell-free system. Carbohydr. Polym. 136, 908-916. doi: 10.1016/j.carbpol.2015.10.010
    Wang, H. , Liu, Y. , Qi, Z. , Wang, S. Y. , Liu S. X. , Li, X. , Wang, H. J. , Xia, X. C. , 2013. An overview on natural polysaccharides with antioxidant properties. Curr. Med. Chem. 20, 2899-2913. doi: 10.2174/0929867311320230006
    Wang, L. , Hu, S. M. , Ullah, M. W. , Li, X. H. , Shi, Z. J. , Yang, G. , 2020. Enhanced cell proliferation by electrical stimulation based on electroactive regenerated bacterial cellulose hydrogels. Carbohydr. Polym. 249, 116829. doi: 10.1016/j.carbpol.2020.116829
    Yang, J. C. , Wang, L. , Zhang, W. , Sun, Z. , Li, Y. , Yang, M. Z. , Zeng, D. , Peng, B. G. , Zheng, W. F. , Jiang, X. Y. , Yang, G. , 2018. Reverse reconstruction and bioprinting of bacterial cellulose-based functional total intervertebral disc for therapeutic implantation. Small 14, 1702582. doi: 10.1002/smll.201702582
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索


    Article Metrics

    Article views (197) PDF downloads(11) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint