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Inhibitory Effect of a Chinese Quince Seed Peptide on Protein Glycation: A Mechanism Study
Yejun Deng, Xiang Wang, Caihong Zhang, Pujun Xie, Lixin Huang
, Available online  , doi: 10.1016/j.jobab.2023.01.008
Non-enzymatic glycation can cause the formation and accumulation of advanced glycation end products (AGEs), and it poses great threats to human health. It is urgent to search for safe and efficient inhibitors to prevent reducing sugar induced protein glycation. In this study, we investigated the anti-glycation activity and mechanism of an identified peptide, Asparagine-Tyrosine-Arginine-Arginine-Glutamic acid (NYRRE) from Chinese quince seed protein hydrolysate, by multispectroscopy, confocal imaging, and computational molecular simulation. Firstly, it was found that NYRRE could scavenge hydroxyl radicals and chelate Fe2+. Besides, the NYRRE was effective in every stage of fructose induced bovine serum albumin (BSA) glycation. The NYRRE could reduce the formation of fructosamine, carbonyl compounds, glycoxidation products and β-amyloid structure. Meanwhile, NYRRE could protect thiol groups and stabilize the spatial conformation of BSA. The NYRRE presented strong inhibition in fluorescent AGEs, and 68.19% of total AGEs formation was prevented with NYRRE at 15 mmol/L. The results of molecular simulation indicated that NYRRE could insert into the hydrophobic pocket of BSA and interact with hot spots, including arginine and lysine residues. The mechanism of NYRRE inhibiting protein glycation could be due to its antioxidant activity, BSA structure stabilizing ability, and specific bond with glycation sites of BSA. These results provided a valuable reference for developing NYRRE as an efficient antiglycation agent in preventing glycation-mediated diseases.
Pichia as Yeast Cell Factory for Production of Industrially Important Bio-Products: Current Trends, Challenges, and Future Prospects
Akansha Shrivastava, Mamta Pal, Rakesh Kumar Sharma
, Available online  , doi: 10.1016/j.jobab.2023.01.007
Yeast has been used as a cell factory for thousands of years to produce a wide variety of complex biofuels, bioproducts, biochemicals, food ingredients, and pharmaceuticals. For a variety of biotechnological production hosts, a few specific genera of yeast have proven themselves. Rapid developments in metabolic engineering and synthetic biology provide a workable long-term supply solution for these substances. In this review, we have covered recent advances in the design of yeast cell factories for the synthesis of terpenoids, alkaloids, phenylpropanoids, and other natural chemicals, primarily focusing on Pichia species. Cutting-edge solutions involving genetic and process engineering have also been discussed. Overall, the review summarized recent advancements and challenges in synthetic and systems biology, as well as initiatives in metabolic engineering aimed at commercializing non-conventional yeasts like Pichia. The processes used in non-traditional yeasts to produce enzymes, therapeutic proteins, lipids, and metabolic products for industrial applications were thoroughly elaborated.
Antimicrobial and Antibiofilm Activities of Pomegranate Peel Phenolic Compounds: Varietal Screening Through a Multivariate Approach
Amira Salim, Pierfrancesco Deiana, Francesco Fancello, Maria Giovanna Molinu, Mario Santona, Severino Zara
, Available online  , doi: 10.1016/j.jobab.2023.01.006
Super-Anti-Freezing, Tough and Adhesive Titanium Carbide and L-Ornithine-Enhanced Hydrogels
Zhangkang Li, Jamie LeBla nc, Hitendra Kumar, Hongguang Zhang, Weijun Yang, Xiao He, Qingye Lu, Jeffrey Van Humbeck, Keekyoung Kim, Jinguang Hu
, Available online  , doi: 10.1016/j.jobab.2023.01.005
In-situ Polymerization of Lignocelluloses of Autohydrolysis Process with Acrylamide
Haosong Zhao, Weijue Gao, Pedram Fatehi
, Available online  , doi: 10.1016/j.jobab.2023.01.004
Efficient Synthesis of Alkyl Levulinates Fuel Additives Using Sulfonic Acid Functionalized Polystyrene Coated Coal Fly Ash Catalyst
Yi Tian, Xiaoting Zhu, Shuolin Zhou, Wenguang Zhao, Qiong Xu, Xianxiang Liu
, Available online  , doi: 10.1016/j.jobab.2023.01.003
Papaya Peel Waste Carbon Dots/Reduced Graphene Oxide Nanocomposite: from Photocatalytic Decomposition of Methylene Blue to Antimicrobial Activity
Hesam Salimi Shahraki, Rani Bushra, Nimra Shakeel, Anees Ahmad, Quratulen, Mehraj Ahmad, Christos Ritzoulis
, Available online  , doi: 10.1016/j.jobab.2023.01.009
Carbon dots (CDs) have gained unprecedented attention as a novel luminescent zero-dimensional carbon nanomaterial owing to their diverse industrial applications. Herein, we reported the sustainable synthesis of fluorescent CDs from papaya peel waste, acting as a natural carbon originator. As-prepared CDs and reduced graphene oxide (RGO) were fabricated in the composites through a facile one-step hydrothermal method. Synthesized RGO/CDs (RC) nanocomposites were characterized using spectroscopic, diffraction, and electron-microscopic techniques. Nanocomposites with variable RGO to CD mass ratios were tested for photodegradation of textile dye methylene blue (MB). The highest photocatalytic activity (degradation efficiency of 87% in 135 min) was obtained in the nanocomposite containing a 2꞉1 mass ratio (RC2). The RGO sheets in the nanocomposite acted as media for electron acceptors, promoting the fast transfer and separation of photoinduced electrons during CDs excitation, thus preventing the recombination of the electron and holes. Based on the agar well diffusion assay, the nanocomposites exhibited excellent antibacterial activity than other tested materials against Bacillus subtilis (Gram-positive) and Pseudomonas aeruginosa (Gram-negative) bacterium. The largest inhibition zone area (22 mm), i.e., the highest antimicrobial activity, was obtained in the nanocomposite tested against Gram-positive strains. Taken together, the synergistic effect of RGO and CDs enhanced the photocatalytic and antibacterial performance of synthesized nanocomposite material.