Volume 8 Issue 3
Jul.  2023
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Peifu Kong, Junichi Peter Abe, Shunsuke Masuo, Toshiharu Enomae. Preparation and characterization of tea tree oil-β-cyclodextrin microcapsules with super-high encapsulation efficiency[J]. Journal of Bioresources and Bioproducts, 2023, 8(3): 224-234. doi: 10.1016/j.jobab.2023.03.004
Citation: Peifu Kong, Junichi Peter Abe, Shunsuke Masuo, Toshiharu Enomae. Preparation and characterization of tea tree oil-β-cyclodextrin microcapsules with super-high encapsulation efficiency[J]. Journal of Bioresources and Bioproducts, 2023, 8(3): 224-234. doi: 10.1016/j.jobab.2023.03.004

Preparation and characterization of tea tree oil-β-cyclodextrin microcapsules with super-high encapsulation efficiency

doi: 10.1016/j.jobab.2023.03.004
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  • This study aimed to prepare tea tree oil-β-cyclodextrin microcapsules using an optimized co-precipitated method. The impact of the volume fraction of ethanol in the solvent system for microencapsulation on encapsulation efficiency was investigated and analyzed sophisticatedly. Super-high encapsulation efficiency was achieved when a 40% volume fraction of ethanol was used for the microencapsulation procedure, where the recovery yield of microcapsules and the embedding fraction of tea tree oil in microcapsules were as high as 88.3% and 94.3%, respectively. Additionally, considering the operation cost, including time and energy consumption, an economical preparation was validated so that it would be viable for large-scale production. Based on the results of morphological and X-ray diffraction analysis, the crystal structure appeared to differ before and after microencapsulation. The results of gas chromatography-mass spectrometry and Fourier transform infrared spectroscopy confirmed the successful formation of microcapsules. Furthermore, the antibacterial activity of the fabricated microcapsules was assessed by a simple growth inhibition test using Bacillus subtilis as the study object, and the hydrophilic property was proved by a water contact angle measurement.


  • There are no conflicts to declare.
    Declaration of Competing Interest
    Supplementary materials
    Supplementary material associated with this article can be found, in the online version, at doi:10.1016/j.jobab.2023.03.004.
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