Improving antioxidant activities of water-soluble lignin-carbohydrate complex isolated from wheat stalk through prolonging ball-milling pretreatment and homogeneous extraction

Di Xie; Zhulan Liu; Yunfeng Cao; Sheng-I Yang; Chen Su; Mi Li

doi:10.1016/j.jobab.2023.11.002

Volume 9 Issue 1

Feb. 2024

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Di Xie, Zhulan Liu, Yunfeng Cao, Sheng-I Yang, Chen Su, Mi Li. Improving antioxidant activities of water-soluble lignin-carbohydrate complex isolated from wheat stalk through prolonging ball-milling pretreatment and homogeneous extraction[J]. Journal of Bioresources and Bioproducts, 2024, 9(1): 113-125. doi: 10.1016/j.jobab.2023.11.002

Citation:

Di Xie, Zhulan Liu, Yunfeng Cao, Sheng-I Yang, Chen Su, Mi Li. Improving antioxidant activities of water-soluble lignin-carbohydrate complex isolated from wheat stalk through prolonging ball-milling pretreatment and homogeneous extraction[J]. Journal of Bioresources and Bioproducts, 2024, 9(1): 113-125. doi: 10.1016/j.jobab.2023.11.002

Citation:

Di Xie, Zhulan Liu, Yunfeng Cao, Sheng-I Yang, Chen Su, Mi Li. Improving antioxidant activities of water-soluble lignin-carbohydrate complex isolated from wheat stalk through prolonging ball-milling pretreatment and homogeneous extraction[J]. Journal of Bioresources and Bioproducts, 2024, 9(1): 113-125. doi: 10.1016/j.jobab.2023.11.002

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Improving antioxidant activities of water-soluble lignin-carbohydrate complex isolated from wheat stalk through prolonging ball-milling pretreatment and homogeneous extraction

doi: 10.1016/j.jobab.2023.11.002

Di Xie^a,b,c,
Zhulan Liu^{a,b,d
,
,},
Yunfeng Cao^a,b,
Sheng-I Yang^e,
Chen Su^a,b,f,
Mi Li^{c
,
,}

a. Jiangsu Co-innovation Center for Efficient Processing and Utilization of Forest Products, Nanjing Forestry University, Nanjing 210037, China;
b. International Innovation Center for Forest Chemicals and Materials, Nanjing Forestry University, Nanjing 210037, China;
c. Center for Renewable Carbon, School of Natural Resources, University of Tennessee, Knoxville, TN 37996, USA;
d. Shandong Huatai Paper Co., Ltd., Dongying 257000, China;
e. School of Natural Resources, University of Tennessee, Knoxville, TN 37996, USA;
f. Key Lab of Biomass Energy and Material, Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Jiangsu Province, Nanjing 210042, China

Funds:

We are grateful for the financial support by National Natural Science Foundation of China (No. 31670591)

Natural Science Foundation of Jiangsu Province (No. BK20160928). This work is also partially supported by the South Eastern Regional Sun Grant Centre and AgResearch at the University of Tennessee.

the fellowship of China Postdoctoral Science Foundation (No. 2022M711229)

Available Online: 2024-01-31
Publish Date: 2023-11-16

Abstract

Abstract

Water-soluble lignin-carbohydrate complex (LCC) rich in polysaccharides exhibits benign in vitro antioxidant activities and distinguishes high biocompatibility from lignin-rich LCC and lignin. However, the antioxidant activity of water-soluble LCCs remains to be improved and its structure-antioxidant relationship is still uncertain. Herein, structurally diversified water-soluble LCCs were isolated under different ball-milling pretreatment durations (4, 6, 8 h), extraction pathways (homogeneous and heterogeneous), and isolation routines (water extracts and residues after water extraction). Their structures were characterized by wet chemistry, chromatography and spectroscopies. Antioxidant activities were evaluated by ferric reducing antioxidant power and 1,1-diphenyl-2-picrylhydrazyl radicals scavenging rate (R_DPPH). Results show that altering ball-milling duration and isolation procedures cause varied structures and antioxidant activities of the water-soluble LCCs. Specifically, prolonging ball-milling duration to 8 hours and homogeneous extraction can enhance their antioxidant activity through releasing more phenolic structures and promoting the extraction of high-molecular-weight LCCs via reducing mass-transfer resistance, respectively. As a result, the R_DPPH of water-soluble LCCs reaches up to 97.35%, which is associated with the arabinan content with statistical significance (P < 0.05). This study provides new insights into the structure-antioxidation relationship of herbaceous LCC as potential antioxidants.
- LCC,
- Homogeneous extraction,
- Ball-milling,
- Structure-antioxidation relationship,
- Wheat straw

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References(60)

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