Volume 9 Issue 1
Feb.  2024
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Bianjing Sun, Ping Wang, Jingang Zhang, Jianbin Lin, Lingling Sun, Xiaokun Wang, Chuntao Chen, Dongping Sun. In situ biosynthesis of bacterial cellulose hydrogel spheroids with tunable dimensions[J]. Journal of Bioresources and Bioproducts, 2024, 9(1): 90-101. doi: 10.1016/j.jobab.2023.12.003
Citation: Bianjing Sun, Ping Wang, Jingang Zhang, Jianbin Lin, Lingling Sun, Xiaokun Wang, Chuntao Chen, Dongping Sun. In situ biosynthesis of bacterial cellulose hydrogel spheroids with tunable dimensions[J]. Journal of Bioresources and Bioproducts, 2024, 9(1): 90-101. doi: 10.1016/j.jobab.2023.12.003

In situ biosynthesis of bacterial cellulose hydrogel spheroids with tunable dimensions

doi: 10.1016/j.jobab.2023.12.003

The authors acknowledge financial support from National Natural Science Foundation of China (No. 51803092

No. 51873087), Fundamental Research Funds for the Central Universities (No. 30920130121001), and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD, China), project funded by Jiangsu Funding Program for Excellent Postdoctoral Talent.

  • Available Online: 2024-01-31
  • Publish Date: 2023-12-26
  • Bacterial cellulose (BC) hydrogel spheroid plays a significant role in diverse fields due to its spatial 3D structure and properties. In the present work, a series of BC spheroids with controllable size and shape was obtained via an in situ biosynthesis. Crucial factors for fabricating BC spheroid including inoculum concentration of 1.35 × 103 CFU/mL, shaking speeds at 100 r/min, and 48-96 h incubation time during the biosynthetic process, were comprehensively established. An operable mechanism model for tuning the size of BC spheroids from 0.4 to 5.0 mm was proposed with a fresh feeding medium strategy of dynamic culture. The resulting BC spheroids exhibit an interactive 3D network of nanofibers, a crystallinity index of 72.3 %, a specific surface area of 91.2 m2/g, and good cytocompatibility. This study reinforces the understanding of BC spheroid formation and explores new horizons for the design of BC spheroids-derived functional matrix materials for medical care.


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