Valorization of the <i>Caragana</i> waste via two-stage bioaugmentation: Optimizing nutrition composition, palatability, and microbial contaminant control

Guilin Du; Marina Ti&#353;ma; Beiru He; Xinghui Zhai; Chenyang Yuan; Zhengding Su; Jiping Shi; Baoguo Zhang

doi:10.1016/j.jobab.2024.07.004

Volume 9 Issue 4

Nov. 2024

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Guilin Du, Marina Tišma, Beiru He, Xinghui Zhai, Chenyang Yuan, Zhengding Su, Jiping Shi, Baoguo Zhang. Valorization of the Caragana waste via two-stage bioaugmentation: Optimizing nutrition composition, palatability, and microbial contaminant control[J]. Journal of Bioresources and Bioproducts, 2024, 9(4): 518-533. doi: 10.1016/j.jobab.2024.07.004

Citation:

Guilin Du, Marina Tišma, Beiru He, Xinghui Zhai, Chenyang Yuan, Zhengding Su, Jiping Shi, Baoguo Zhang. Valorization of the Caragana waste via two-stage bioaugmentation: Optimizing nutrition composition, palatability, and microbial contaminant control[J]. Journal of Bioresources and Bioproducts, 2024, 9(4): 518-533. doi: 10.1016/j.jobab.2024.07.004

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Valorization of the Caragana waste via two-stage bioaugmentation: Optimizing nutrition composition, palatability, and microbial contaminant control

doi: 10.1016/j.jobab.2024.07.004

Guilin Du^a,b,c,
Marina Tišma^d,
Beiru He^a,b,c,
Xinghui Zhai^a,b,c,
Chenyang Yuan^a,b,c,
Zhengding Su^{e
,
,},
Jiping Shi^a,b,c,
Baoguo Zhang^{a,b
,
,}

a. Laboratory of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China;
b. University of Chinese Academy of Sciences, Beijing 100049, China;
c. School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China;
d. Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 18, Osijek HR-31000, Croatia;
e. School of Pharmaceutical Sciences and Institute of Materia Medica, Xinjiang University, Urumqi 830017, China

Funds:

ma would like to thank the Chinese Academy of Sciences for the award of aPresident's International Fellowship Initiative(No.2024PVA0097).

This study was financed by National Key R&D Program of China(No.2017YFE0112700) and Binzhou Institute of Technology(No.GYY-NYHJ-2023-WT-001). The authors would like to thank Shiyanjia Lab (www.shiyanjia.com) for the electronic tongue analysis. Marina Tiš

Available Online: 2024-10-26
Publish Date: 2024-07-16

Abstract

Abstract

Caragana korshinskii kom. (CKK) waste, a common forestry byproduct in northwest of China, presents challenges in its transformation into alternative ruminant feed due to its initial nutritional limitations and unappealing palatability. Conventional strategies, such as ensiling and fungal-based solid-state fermentation (SSF) cannot effectively address this issue in practice. Herein, a two-stage bioaugmentation (TBA) process was devised, leveraging the benefits of ensiling and SSF. During the anaerobic ensiling phase, CKK waste was inoculated with Lactiplantibacillus plantarum LP1, effectively suppressing potential animal pathogens such as Aspergillus and Nocardiopsis while enriching the material with potential probiotics like Pediococcus and Lactiplantibacillus, reaching an abundance of 95.7%. In the subsequent aerobic SSF stage, the ensiled CKK underwent inoculation with the white-rot fungus Irpex lacteus F17, which became enriched to 87.9%. Comprehensive multi-omics analysis identified Irpex as the key taxon, possessing an extensive redox enzyme system that led to the improvement in nutrient composition, reduction of astringent phenolic substances, and mitigation of mycotoxins. As a result, the crude protein content of the CKK increased by 39.2%, while lignin, total phenolic substances, and tannic acid content decreased by 24.4%, 52.2%, and 51.4%, respectively. The mycotoxin levels, including aflatoxin B₁, zearalenone, and vomitoxin, were rendered negligible, confirming the safety. Overall, this study demonstrates the TBA strategy can successfully transform challenging and unpalatable CKK waste into a nutrient-enriched and safe mycelium-based bioproduct, thereby enabling the valorization of a previously underutilized forestry resource as a promising alternative feed.
- Caragana waste,
- Two-stage bioaugmentation,
- Alternative feed,
- Anti-nutritional factor,
- Mycotoxin

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References(65)

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