Immobilized enzyme microreactor system with bamboo-based cellulose nanofibers for efficient biotransformation of phytochemicals

Quan Zhou; Zijing Zhao; Litao Wang; Jiandong Wang; Lina Fu; Jihong Cui; Guosheng Liu; Jie Yang; Yujie Fu

doi:10.1016/j.jobab.2025.03.004

Volume 10 Issue 2

May 2025

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Article Navigation > Journal of Bioresources and Bioproducts > 2025 > 10(2): 224-238

Quan Zhou, Zijing Zhao, Litao Wang, Jiandong Wang, Lina Fu, Jihong Cui, Guosheng Liu, Jie Yang, Yujie Fu. Immobilized enzyme microreactor system with bamboo-based cellulose nanofibers for efficient biotransformation of phytochemicals[J]. Journal of Bioresources and Bioproducts, 2025, 10(2): 224-238. doi: 10.1016/j.jobab.2025.03.004

Citation:

Quan Zhou, Zijing Zhao, Litao Wang, Jiandong Wang, Lina Fu, Jihong Cui, Guosheng Liu, Jie Yang, Yujie Fu. Immobilized enzyme microreactor system with bamboo-based cellulose nanofibers for efficient biotransformation of phytochemicals[J]. Journal of Bioresources and Bioproducts, 2025, 10(2): 224-238. doi: 10.1016/j.jobab.2025.03.004

Citation:

Quan Zhou, Zijing Zhao, Litao Wang, Jiandong Wang, Lina Fu, Jihong Cui, Guosheng Liu, Jie Yang, Yujie Fu. Immobilized enzyme microreactor system with bamboo-based cellulose nanofibers for efficient biotransformation of phytochemicals[J]. Journal of Bioresources and Bioproducts, 2025, 10(2): 224-238. doi: 10.1016/j.jobab.2025.03.004

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Immobilized enzyme microreactor system with bamboo-based cellulose nanofibers for efficient biotransformation of phytochemicals

doi: 10.1016/j.jobab.2025.03.004

Quan Zhou^a,
Zijing Zhao^a,
Litao Wang^a,b,c,
Jiandong Wang^a,
Lina Fu^a,
Jihong Cui^a,
Guosheng Liu^a,
Jie Yang^{a,b
,
,},
Yujie Fu^{a,b,c
,
,}

a. State Key Laboratory of Efficient Production of Forest Resources, Beijing Forestry University, Beijing 100083, China;
b. The College of Forestry, Beijing Forestry University, Beijing 100083, China;
c. The Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing 100083, China

Funds:

This work was supported by National Natural Science Foundation of China (No. 32271805), National Science and Technology Innovation Project (No. 2022XACX1100), National Key R&D Program of China (No. 2022YFD2200602), the 111 Center (No. B20088) and 5·5 Engineering Research & Innovation Team Project of Beijing Forestry University (No. BLRC2023A01).

Available Online: 2025-05-09
Publish Date: 2025-03-28

Abstract

Abstract

The enzyme immobilization technique has been widely applied in biotechnology, biomedicine, and environmental remediation. In this research, carboxylated bamboo-based cellulose nanofibers (BCNFs) were obtained by one-step oxidation modification of bamboo fibers using ammonium persulphate. The surface carboxyl groups of the BCNFs were modified by a crosslinking agent and then combined with polyethylene imine (PEI) functionalized magnetic nanoparticles to construct a microreactor system for enzyme loading by the methods of electrostatic self-assembly and physical adsorption. Contrasted with free β-glucosidase, the microreactor possesses higher relative enzyme activity at pH 5.5 and 50 °C, and the storage stability is significantly higher, with >75% relative enzyme activity after storage at 4 °C for 15 d. In addition, the β-glucosidase loaded on the microreactor facilitates its separation from the reaction medium and subsequent reuse. After completing five cycles of use, it retained 76.47% of its initial activity. The biotransformation of geniposide reached 93.10%, and the genipin concentration increased 1.2 folds higher than that in the original plant extract. Therefore, PEI@Fe₃O₄@BCNFs microreactor immobilized with β-glucosidase can be successfully used to produce higher activity aglucone such as genipin from geniposide, and it might also have the potential to convert phytochemicals by the immobilized enzyme microreactor system with bamboo-based cellulose nanofibers in the natural production field.
- Microreactor,
- Bamboo fiber,
- Cellulose nanofiber,
- Enzyme immobilization,
- Biotransformation

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References(65)

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